血红蛋白模拟辣根过氧化物酶在酶联免疫分析中的应用

血红蛋白模拟辣根过氧化物酶在酶联免疫分析中的应用 Application of Hb as the Mimetic Labeling Enzymeof HRP in the Immunoassay 李得加 LI De-jia 1 2 first-author 邹国林 ZOU Guo-lin 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 提出了以血红蛋白（Hb）作为辣根过氧化物酶模拟酶,用碳二亚胺法将其标记人IgG,Hb标记人IgG在形成免疫复合物后,仍可保持其95%的催化活性.以邻苯二胺作为氢供体,以羊抗人IgG为分析模型,建立了以血红蛋白作为酶标记物的竞争型免疫分析新体系.人IgG在15～1000μg/L范围内与体系吸光度呈良好的线性关系,线性方程为A=0.297-1.286E-4[IgG]（μg/L）,相关系数r=0.9992（n=8）,检测限为15μg/L.用于人正常血清中人IgG含量的测定,加标回收率为94%～108%,结果令人满意. A carbodiimide method was used to label the human IgG using Hb as the mimetic enzyme of HRP. The immuo-conjugate of Hb with human IgG remained 95% of the catalytic activity of Hb. A new competing type immunoanalytical system was estabilished using Hb as the labeling enzyme, o-phenylenediamine as the hydrogen donor, and the goat anti-human IgG as the analytical model. The concentration of human IgG had a good linear relationship with the absorbance of the system in the concentration range of 15～1 000 μg/L. The linear regression equation A=0.297-1.286E-4\（μg/L）, The correlation coefficient was 0.999 2（n=8）and the determination limit was 15 μg/L. The method was used for determination of IgG in normal human serum with good satisfactory results. The analytical recoveries are 94%～108%. 血红蛋白 酶联免疫分析 碳二亚胺 hemoglobin enzyme-linked immunosorbent assay carbodiimide Q554.6 国家自然科学基金资助项目（39770200） 2003-06-01 2021-04-01 6