碱性蛋白酶高产菌株的选育

碱性蛋白酶高产菌株的选育 The Breeding of Alkaline Protease High-Yielding Strain 陈向东 CHEN Xiang-dong 1 2 first-author 彭珍荣 PENG Zhen-rong 1 2 沈萍 SHEN Ping 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 以利福平抗性及牛奶平板上产生的透明水解圈的大小作为选择标记,用钴60对本实验室筛选获得的碱性蛋白酶高产菌株地衣芽孢杆菌C6进行诱变处理,筛选获得了一株酶产量大幅度提高的菌株L106,经发酵条件优化,其产酶活性稳定在19000U/mL左右,是出发菌株的1.9倍.与出发菌株相比,该菌的发酵周期也有所缩短,而产酶的最适温度和最适pH保持不变,并保持了芽孢形成较少的优良特性,具有良好的实际应用潜力. By mutagenizing with Co 60 -γ-rays and subsequent enzyme production condition optimization, a mutant strain Bacillus licheniformis L106 was obtained from B. licheniformis C6. Around 19 000 U/mL of the enzyme productivity could usually be achieved by the strain, which was 1.9 fold higher than that achieved by the strain C6. Compared to the start strain C6, L106 has higher substantial application potential since it shortened the enzyme production period while still kept the merit of forming less spores during enzyme fermentation, and the optimal reaction pH and temperature of the produced enzyme were also unchanged. 碱性蛋白酶 地衣芽孢杆菌 诱变 alkaline protease Bacillus licheniformis mutagenesis Q933 2003-06-01 2021-04-01 6