一氧化碳血红素C系列化合物的共振拉曼光谱

一氧化碳血红素C系列化合物的共振拉曼光谱 RESONANCE RAMAN SPECTROSCOPY OF CARBONMONOXY HEME C SYSTEMS 何季平 He Jiping 1 2 first-author 尤乃亭 1 2 林顺华 1 2 武汉大学分析测试中心 武汉大学分析测试中心 Centre of Analysis and Testing,Wuhan University,China)Yu Naiteng Lin Shunhua Centre of Analysis and Testing,Wuhan University,China)Yu Naiteng Lin Shunhua 美国佐治亚理工学院化学系 美国佐治亚理工学院化学系 本文报告了血红素 C 系列化合物(H 8pt ,H 11 ,H 65 )一氧化碳络合物的共振拉曼光谱伸展振动频率。发现随着一氧化碳结合亲和力的减小,其 v（F-co）伸展振动频率逐渐增加,而它们的 v（C-O）伸展振动频率相应地减小。但在酸性溶液中（pH2.0）,这三种血红素化合物的 v（F e -CO）伸展振动频率(531cm -1 )和 v（C-O）(1970cm -1 )彼此相近。我们同时列出了一氧化碳血红素 C 系列化合物氧化状态和自旋状态特征拉曼频率。一般情况下,一氧化碳血红素C系列化合物呈现低自旋状态,但在酸性溶液中产生中间自旋状态或高自旋状态及其混合态。 We report resonance Raman detection of v （Fe-CO） and v （c-o） stretching fr- equencies in the CO complexes of heme C system(H 8 pt,H 11 ,H 85 ).It was found that the v （Fe-co） stretching frequencies increases with decreasing CO binding affinity.The v （Fe-CO） stretching frequency of the H 8 pt—CO(498cm -1 ), H 65 —CO(502cm -1 )and H 11 —CO(509cm -1 )increases with a decrease in（C—O） stretching frequencies of H 8 pt—CO(1969cm -1 ),H 65 —CO(1961cm -1 )and H 11 —CO(1956cm -1 ).But in acid solution（pH2.0）for H 8 pt—CO, H 65 —CO and H 11 —CO,the v （Fe-CO） stretching frequencies are about 531cm -1 .The v （c-o） st- retching frequencies appear at about 1970cm -1 ,they are similar each other. There are several oxitation and spin state sensitive Raman bands that are characterist of particular classes of heme C system.It is noted that the fer- ric heme C system at acid solution（pH 2 .0）generate forms which are typical of high spin ferric hemes and hemoprotein.The carbonmonoxy heme C system usually,appear Low-Spin state,but in acid solution（pH 2 .0）generate interme- diate or high spin form. 血红素 C 系列化合物 共振拉曼光谱 氧化状态 自旋状态 伸展振动频率 heine C system resonance raman spectroscopy oxitation state spin state stretching frequency 1990-03-01 2021-04-01 3