漆树漆酶的催化氧化作用——Ⅲ.底物疏水化对中国漆树漆酶催化活性的影响

漆树漆酶的催化氧化作用——Ⅲ.底物疏水化对中国漆树漆酶催化活性的影响 CATALYZED OXIDATION BY RHUS VERNICIFERA LACCASE Ⅲ.Influence of hydrophobicity of Substrates on cataytic activity of Rhus vernicifcra laccase from Chinese laqquer 王光辉 Wang Guanghui Cai Qiande Chen Cheng Yang Zheniping 1 first-author 蔡乾德 1 沈(王争) 1 杨振平 1 武汉大学化学系 武汉大学化学系 Department of Chemistry,Wuhan University Department of Chemistry,Wuhan University 用分光光度法考察了原儿茶酸的甲酯、正丁酯、正戊酯、正辛酯和正十二烷基酯在0.1mol 磷酸缓冲液（pH8.0）-二甘醇混合溶剂（V:V=1:1）中,用竹溪漆树漆酶酶催下的氧化动力学行为,找出了动力学参数与酯中烷基链长的依存关系。结果表明,不为漆酶所喜爱的亲水性底物（原儿茶酸）,经过适当疏水化处理,可以有效地改善其反应性和提高漆酶的催化活性。 The kinetic property of oxidation of methyl.n-butyl、n-amyl、n-octyl and n-dodecyl protocatechuate under catalysis of Rhus vernicifera laccase from Chu-Xi lacquer was measured by a spectraphotometric method in 0.1M phos phate buffer（pH8.0）-diglycol（1:Ⅳ/Ⅴ）,and relationship between the kinetic pa- rameter and chain length of esters was observed.These results show that the reactivity of some nonprefererred hydrophilic substrates and the catalytic acti- vity of Rhus vernicifera laccase can be improved efficiently,through the suit able hydrophobic processing in certain site of these substrates. 漆树漆酶 原儿茶酸酯 底物疏水化 催化活件 分光光度法 rhus vernicifera laccase protocatechuate hydrophobicity of substrates catalytic activity spectrophotometric method 1991-01-01 2021-04-01 1