产黑色素B.thuringiensis重组菌株的构建及其培养条件的优化

产黑色素B.thuringiensis重组菌株的构建及其培养条件的优化 Construction of the Bacillus Thuringiensis Recombinant Strain with Melanin Formation and Research on the Condition of Producing Melanin 阮丽芳 RUAN Li-fang 1 2 first-author 王玉洁 WANG Yu-jie 1 2 沈萍 SHEN Ping 1 2 武汉大学生命科学院 武汉大学生命科学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学院,湖北,武汉,430072 武汉大学生命科学院,湖北,武汉,430072 将嗜麦芽假单胞菌中产生黑色素的基因 （melgene）克隆到穿梭载体 pHT3 10 1中 ,并将它处于表达系统cry3A的控制下 ,构建得到重组质粒 pHTAM .将此重组质粒用电脉冲的方法转入苏云金芽胞杆菌受体菌株BMB171中 ,得到重组菌株RSA .研究结果表明 ,处于cry3A控制下mel基因在BMB171菌株中得到了成功的表达 .本研究进一步采用红外光谱扫描法检测RSA所产黑色素的性质 ,并通过改变培养条件来提高黑色素的产量 We subcloned the mel gene from Pseudomonas maltophilia, which is responsible for melanin formation, into shuttle vector pHT3101. The mel gene was inserted downstream of cry3A promoter. We transformed the two plasmids into BMB171（a mutant strain of Bacillus thuringiensis which can not produce crystal） and acquired a recombinant strains RSA. The research results revealed that the mel gene expressed under the control of cry3A promoter in BMB171. Furthermore, we detect the character of melanin produced by RSA and improve the yield of melanin by changing the cultural condition. 苏云金芽孢杆菌 mel基因 黑色素 Bacillus thuringiensis mel gene melanin Q933 2003-02-01 2021-04-01 2