鹌鹑和黑斑蛙核糖体蛋白L15 cDNA片段的克隆和序列分析

鹌鹑和黑斑蛙核糖体蛋白L15 cDNA片段的克隆和序列分析 Molecular Cloning and Sequence Analysis of Ribosomal Protein L15 （RPL15） cDNA Fragments Respectively from Coturnix coturnix japonica and Rana nigromaculata 张竞男 ZHANG Jing-nan 1 2 first-author 宋平 SONG Ping 1 2 彭茂宇 PENG Mao-yu 1 2 吕道远 LU Dao-yuan 1 2 陈云贵 CHEN Yun-gui 1 2 周伟 ZHOU Wei 1 2 陈春红 CHEN Chun-hong 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 通过RT PCR方法,分别从两栖类的黑斑蛙（Rananigromaculata）和鸟类的鹌鹑（Coturnixcoturnixjaponica）中首次克隆了核糖体大亚基蛋白L15（RPL15,ribosomalproteinL15）的cDNA片段,并分析了其序列特征.黑斑蛙和鹌鹑的rpl15cDNA片段分别为467个核苷酸和408个核苷酸,推测分别编码155个氨基酸和136个氨基酸的蛋白片段,与其它已知的脊椎动物rpl15序列有高度的同源性.应用这两条cDNA序列和其它已知的脊椎动物rpl15序列构建的系统发育树显示聚类结果与传统分类一致. The cDNA fragments of ribosomal protein L15 （RPL15） were cloned respectively from Rana nigromaculata and Coturnix coturnix japonica by RT-PCR. The two cDNA fragments are individually composed of 467 bp and 408 bp, encoding a protein fragment of 155 aa and another one of 136 aa. Alignment analysis indicated that both the two nucleotide sequences and the deduced amino acid sequences show high identity to other known rpl15 sequences from vertebrates. The two cDNA sequences were used to construct phylogenetic trees with other known vertebrate rpl15 sequences, and the obtained trees demonstrated similar topology with the classical systematics. 黑斑蛙 鹌鹑 核糖体蛋白L15（RPL15） 序列分析 系统发育树 Rana nigromaculata Coturnix coturnix japonica ribosomal protein L15（RPL15） sequence analysis phylogeny Q953 国家自然科学基金资助项目（30150005） 2003-06-01 2021-04-01 6