醋酸泼尼松龙的荧光分析法研究

醋酸泼尼松龙的荧光分析法研究 Study on Spectrofluorimetry of Prednisolone Acetate 刘义庆 LIU Yi-qing 1 1 2 3 4 first-author 马勇 MA Yong 1 1 2 3 4 张曙盛 ZHANG Shu-sheng 1 1 2 3 4 潘祖亭 PAN Zu-ting 1 1 2 3 4 武汉大学化学与分子科学学院 武汉大学化学与分子科学学院 College of Chemistry and Molecular Sciences,Wuhan University College of Chemistry and Molecular Sciences,Wuhan University 武汉大学化学与分子科学学院,湖北,武汉,430072 武汉大学化学与分子科学学院,湖北,武汉,430072 School of Chemistry and Enviromental Engineering,Jianghan University School of Chemistry and Enviromental Engineering,Jianghan University 江汉大学化学与环境工程学院 江汉大学化学与环境工程学院 ,湖北,武汉,430072 ,湖北,武汉,430072 肾上腺皮质激素类药物醋酸泼尼松龙被浓H2 SO4氧化 ,生成有荧光的反应产物 ,据此建立了一种测量醋酸泼尼松龙的荧光光度分析新方法 ,考察了醇、表面活性剂和 β 环糊精对产物荧光性质的影响 ,提出了醋酸泼尼松龙被氧化及荧光增强的作用机理 ,检出限为 4 .96× 10 -7mol·L-1,荧光强度与醋酸泼尼松龙在 0～ 1.2 4× 10 -5mol·L-1浓度范围内呈良好线性关系 A new method with high sensitivity for the determination of prednisolone acetate was proposed according to the fluorescence character of the product that came from the reaction of prednisolone acetate with concentralic sulfuric acid, the reaction condition and the mechanism were studied as well. The fluorescence intensity was mearsured with excitation wavelengths of 242 nm and emission wavelengths of 398 nm. The results show that the fluorescence intensity of the system presents a linear relationship with the concentration of prednisolone acetate in the range from 0 to 1.24×10 -5 mol·L -1.The detection limit is 4.96×10 -7 mol·L -1. 醋酸泼尼松龙 溶剂效应 荧光光度法 药物分析 prednisolone acetate solvent effects spectrofluorimetry pharmaceutical analysis O657.3 国家自然科学基金资助项目 （2 0 2 75 0 2 8） 2003-04-01 2021-04-01 4