大肠杆菌密码子与蛋白质二级结构的关系

大肠杆菌密码子与蛋白质二级结构的关系 Relationship Between the Codon and Protein Secondary Structure in Escherichia coli 冯晶 FENG Jing~ 1 2 first-author 周怀北 1 2 刘娟 1 2 武汉大学计算机学院 武汉大学计算机学院 1,2), ZHOU Huai-bei~ 1,2), ZHOU Huai-bei~ 武汉大学计算机学院 湖北武汉430072武汉大学高科技研究与发展中心湖北武汉,430072 武汉大学计算机学院 湖北武汉430072武汉大学高科技研究与发展中心湖北武汉,430072 通过对大肠杆菌（E.coli）的蛋白质二级结构α螺旋、β折叠和无规卷曲的片段分别进行分析,发现有些蛋白质二级结构所对应氨基酸的密码子在结构片段的不同位置上具有明显的分布偏向性.有些密码子偏向于出现在结构片段的头部,有些则偏向于出现在结构片段的中部或尾部.同时还发现α螺旋片段中具有位置偏向性的密码子较多,而β折叠和无规卷曲片段中具有偏向性的密码子则较少.对人类蛋白质二级结构的片段进行统计分析,发现密码子也具有类似的偏向性,但是与大肠杆菌相比,又具有自己的特征. The source data used in our study are downloaded from the public databases, PDB, GenBank and Swiss-Prot. First of all, the sequences of protein secondary structure and its corresponding DNA are extracted from the source data by aligning the amino acid sequences of the proteins. Then, the relationship between protein secondary structures and the DNA sequences is constructed successfully. It is found that some codons are strongly correlated to some special part of structure segments by analyzing the segments of E.coli’s protein secondary structure （α-Helix,β-Sheet and Coil）. Some codons appear in the head of the segment frequently, and on the contrary some codons are connected to the middle or tail part of the segments. On the other hand, the quantity of the bias codons in α-Helix segments is much more than it in β\|Sheet and Coil. At the same time, we also find the codon bias in Human proteins by analyzing the segments of protein secondary structures. 密码子 蛋白质二级结构 大肠杆菌 codon protein secondary structure Escherichia coli Q936 国家自然科学基金资助项目（60301009）;留学回国人员科研启动基金资助项目;武汉市青年晨光计划项目 2004-04-01 2021-04-01 4