荧光光谱法研究枸橼酸氯米芬与DNA的相互作用

荧光光谱法研究枸橼酸氯米芬与DNA的相互作用 Interaction of DNA and Clomifene Citrate by Fluorescence Spectrum 潘祖亭 PAN Zu-ting 1 2 first-author 余军平 YU Jun-ping 1 2 武汉大学化学与分子科学学院 武汉大学化学与分子科学学院 College of Chemistry and Molecular Sciences, Wuhan University College of Chemistry and Molecular Sciences, Wuhan University 武汉大学化学与分子科学学院,湖北,武汉,430072 武汉大学化学与分子科学学院,湖北,武汉,430072 以溴化乙锭为荧光探针,利用荧光光谱法研究了枸橼酸氯米芬与脱氧核糖核酸（DNA）之间的相互作用.结果表明,在pH=7.4的模拟体液条件下,枸橼酸氯米芬与DNA可发生相互作用,但作用较弱.当枸橼酸氯米芬浓度较低（≤1.00×10-4mol/L）时,主要作用方式表现为沟槽方式;当枸橼酸氯米芬浓度较高时,主要作用方式表现为混合模式. The interaction between clomifene citrate（CC） and deoxyribonucleic acid （DNA） was studied by fluorescence spectroscopy using ethidium bromide （EB） as a fluorescence probe. It is showed that at Tris-HCl （pH 7.4）, CC can react with DNA although the interaction was weak. The major mode of recognition was groove binding between the CC and DNA when the concentration was low(≤1.00×10 -4 mol/L). The recognition mode was a mixture of grooving and intercalating when the concentration was higher, perhaps some of CC intercalates into the helix of DNA. 荧光光谱法 脱氧核糖核酸 枸橼酸氯米芬 fluorescence spectrum deoxyribonucleic acid clomifene citrate O657.39 国家自然科学基金资助项目（20275028） 2004-04-01 2021-04-01 4