修饰聚合物液固亲和萃取体系纯化大豆中的组氨酸

修饰聚合物液固亲和萃取体系纯化大豆中的组氨酸 Purification of Histidine in Soya Bean by Using the Modified Polymer Liquid-Solid Affinity Extraction System 李逢雨 LI Feng-yu 1 2 first-author 孙小梅 SUN Xiao-mei 1 2 李步海 LI Bu-hai 1 2 中南民族大学化学与生命科学学院 中南民族大学化学与生命科学学院 College of Chemistry and Life Science, South-Central University for Nationalities College of Chemistry and Life Science, South-Central University for Nationalities 中南民族大学化学与生命科学学院,湖北,武汉,430074 中南民族大学化学与生命科学学院,湖北,武汉,430074 聚乙二醇（PEG）6000经亚氨基二乙酸（IDA）修饰后和CuSO4反应,形成PEG修饰聚合物PEG （IDA Cu）2,与吐温80、磷酸盐混合,构成液 固亲和萃取体系,直接从大豆蛋白匀浆中提取氨基酸.选定萃取条件为:磷酸盐摩尔比n（K2HPO4）∶n（NaH2PO4）为4.8∶1,体系pH值7.70,总盐浓度为1.60mol·L-1;吐温80的体积分数为10.5%.结果表明:该体系对大豆蛋白匀浆中氨基酸的二次萃取率为66.5%,用离子交换技术后继处理,得纯度较高的组氨酸. The polyethylene glycol（PEG） 6000 modified with iminodiacetic acid（IDA） reacted with （CuSO 4 ） to form the modified polymer complex PEG-（IDA-Cu） 2 .The polymer-salt-H 2 O liquid-solid affinity extraction system was composed by PEG-（IDA-Cu） 2 with tween 80 and phosphate. It was used to extract amino-acids from the soya bean homogenate directly. The optimal conditions are as follows: the phosphate concentration is 1.6 mol·L -1 ; the mole ratio between K 2 HPO 4 and NaH 2 PO 4 is 4.8∶1, the system pH is 7.70; The tween 80 concentration is 10.5%; The percentage of two-step extraction is 66.5%. The higher purity histidine was obtained after the treatment with the ion exchange technology. PEG修饰聚合物 液固亲和萃取体系 组氨酸 大豆 modified polymer PEG liquid-solid affinity extraction system histidine soya bean O657.15 国家自然科学基金资助项目（29475219） 2004-02-01 2021-04-01 2