固相微萃取与气相色谱/质谱法联用分析葫芦巴浸膏的挥发性成分

固相微萃取与气相色谱/质谱法联用分析葫芦巴浸膏的挥发性成分 Analysis of Volatile Components of Fenugreek Concrete by Gas Chromatography/Mass Spectrometry with Solid-Phase Microextraction 董丽 DONG Li~ 1 2 3 4 first-author 蔡凌霜 1 2 3 4 朱书奎 1 2 3 4 吴采樱 1 2 3 4 武汉大学化学与分子科学学院 武汉大学化学与分子科学学院 1,2), CAI Ling-shuang~1, ZHU Shu-kui~1,WU Cai-ying~1 1,2), CAI Ling-shuang~1, ZHU Shu-kui~1,WU Cai-ying~1 武汉大学化学与分子科学学院,湖北,武汉,430072 武汉大学化学与分子科学学院,湖北,武汉,430072 新乡医学院基础部 新乡医学院基础部 ,湖北,武汉,430072 ,湖北,武汉,430072 采用自由基交联和溶胶 凝胶技术,成功制备了乙烯基单苯并 15 冠 5（AB15C5）新型固相微萃取（SPME）涂层.用其对葫芦巴浸膏的挥发性成分进行萃取,用气相色谱/质谱（GC/MS）联用技术进行分离分析,共鉴定出29种化学成分,主要成分有4 乙基苯酚、丁酸乙酯、邻苯二甲酸二丁酯、2,4 双（1,1 二甲基乙基）苯酚、苯甲酸苄酯、9,12 十八碳二烯酸乙酯、二氢 5 苯基 2（3H）呋喃酮、邻苯二甲酸二乙酯等.在固相微萃取实验中对萃取时间、萃取温度、解吸时间、离子强度等实验条件进行了优化,并与商用PA涂层进行了比较. Appling radical cross-linked and sol-gel technique, a novel solid-phase microextraction fiber with 3′-allylbenzo-15-crown-5 was successfully used to extract fenugreek concrete. volatile components were analyzed by GC/MS, and their relative contents determined by area normalization, 29 constituents were identified, which accounted for （91.45%） of concrete. 4-ethyl phenol, ethyl butyrate, dibutyl phthalate, 2,4-bis（1,1-dimethylethyl）phenol, ethyl 9,12-octadecadienoate, dihydro-5-pentyl-2（3H）furanone, etc were the major volatile components in the concrete . In SPME experiments, The factors affecting quantitative results, including absorption time, absorption temperature, ionic strength and desorption time are discussed. Results obtained from different SPME extraction fibers were also compared. SPME with 80 μm crown ether fiber gave the most desirable results. 葫芦巴浸膏 固相微萃取 乙烯基单苯并-15-冠-5 自由基交联 溶胶凝胶 挥发性成分 fenugreek concrete solid phase microextraction 3′-allylbenzo-15-crown-5 radical cross-linked sol-gel volatile components O657.63 国家自然科学基金资助项目（29977013） 2004-02-01 2021-04-01 2