小麦根线粒体可溶性F₁-ATPase的纯化及生化特性研究

小麦根线粒体可溶性F 1 -ATPase的纯化及生化特性研究 PURIFICATION AND CHARACTERIZATION OF THE SOLUBLE F 1 -ATPase FROM WHEAT ROOTS MITOCHONDRIA 王延枝 1 first-author 冷楠 1 武汉大学生物学系 武汉大学生物学系 用氯仿处理小麦根的线粒体,经Sephadex G-200和DEAE-Cellulose柱层析纯化,得到的可溶性F 1 -ATPase和结合在线粒体内膜上的完整ATPase相似之处为:它们都受叠氮化钠和硝酸盐抑制,被HCO 3 - 和C1 - 激活以及对ATP的水解能力大于对GTP的水解能力.两者的不同之处为:可溶性F 1 -ATPase不受寡霉素和二环己基碳二亚胺（DCCD）的抑制。该文报导了简单、快速制备F 1 -ATPase的方法和线粒体F 1 -ATPase的生化特性. The biochemical properties of the soluble F 1 -ATPase of mitochondria from wheat roots were examined to those of the mitochondrial membrane-bound ATPase. The mitochondria were treated with chloroform and purified by Sephadex G-200 and DEAF-Cellulose chromatography. The solube F 1 -AT- Pase is insensitice to oligomycin and DCCD. By both the mitochondria membrane-bound ATPase and the soluble F 1 -ATPase of mitochondria ATP was hydrolyzed in preference to GTP. The soluble F 1 -AT- Pase of mitochondria, like the mitochondrial membrane-bound ATPase, was inhibited by azide, nitrate and NEM, and was stimulated by bicarbonate but not by chloride that was different from the mitochon- drial membrane-bound ATPase. 小麦 线粒体 F1-ATPase mitochondria F1-ATPase wheat Q;Q244;S512.1 国家自然科学基金资助课题 1993-01-01 2021-04-01 1