用大肠杆菌质粒转化嗜麦芽假单胞菌

用大肠杆菌质粒转化嗜麦芽假单胞菌 SDUDIES ON TRANSFORMATION OF PSEUDOMONAS MALTOPIIILIA WITH PLASMID PGPI-2 FROM E.COLI 袁斌 1 first-author 沈萍 1 武汉大学生物学系 武汉大学生物学系 将大肠杆菌的质粒pGP1-2成功地转化进嗜麦芽假单胞菌P27,研究了该菌形成感受态的条件及影响转化效率的各种因素,并比较了pGP1-2质粒在大肠杆菌及嗜麦芽假单胞菌中的转化效率.实验结果指出,处于对数生长后期的嗜麦芽假单胞菌P27经Cacl 2 处理后可接受pGP1-2质粒的转化;其转化频率随CaCl 2 处理时间的延长而提高,并且20mmo1/L Mg ++ 明显地提高转化效率.此外,75mmol/L DTT,100mmol/L KCI,45mmol/L MnCl 2 ,都有利于嗜麦芽假单胞菌的转化.以上研究为建立嗜麦芽假单胞菌的受体-载体系统,进一步开展对该菌的遗传学研究及实际应用打下了基础. The conditions for competence formation in P. maltophilia P27 and factors effecting plasmid trans- formation are described. P. maltophilia P27 was able to transform plasmid pGPI-2 from E. coli after treatment of the cells in late-exponential growth with CaCl 2 . The frequence of transformation was increased with lengthen of time-treaed by CaC1 2 and was promoted by adding 20mmo1/L Mg 2+ . Besides, some of compounds in appropriate concentration, such as 75mmol/L DTT, 100mmol/L KCl, 45mmol/L MnCl 2 , were also beneficial to transformation of P. maltophilia. 质粒 转化 嗜麦芽假单胞菌 Plasmid Transformation P. maltophilia O433 1993-01-01 2021-04-01 1