粘质沙雷氏菌合成碱性蛋白酶的阻遏作用

粘质沙雷氏菌合成碱性蛋白酶的阻遏作用 CATABOLITE REPRESSION OF THE PRODUCTION OF ALKALINE PROTEASE BY SERRATIA MARCESCENS 黄利群 1 first-author 彭珍荣 1 武汉大学生物学系 武汉大学生物学系 用粘质沙雷氏菌（Serratia marcescens）的菌悬液研究了该菌合成碱性蛋白酶的阻遏作用.八种碳源对产酶的影响各不相同,其中以琥珀酸及DL -1 苹果酸的阻遏作用最为显著.琥珀酸对产酶的调节随浓度增大,阻遏作用增强.且加入时间愈早,阻遏作用也愈强.其阻遏机制与利福平类似,与氯霉素则不同.cAMP能消除琥珀酸对产酶的阻遏作用,表明该阻遏作用属分解代谢物的阻遏. Catabolite repression of Serratia marcescens producting alkaline protease was studied by using the cell suspensions. Eight carbon sources affected the production of protease differently. The repression of succinate and DL-malate was most effective among these. When the concentration of succinate in- creased, the repression of protease formation was enhanced. In addition, the repression of succinate on extracellular protease secretion was relative with time of adding it, namely, the earlier, the better. The repression mechanism of succinate was similar to that of rifamycin, but not same as that of chloram- phenicol. cAMP could overcome the reprecssion produced by succinate. The data suggested that exoen- zyme secretion was controlled by a mechanism of catablite repression. 碱性蛋白酶 分解代谢物阻遏 粘质沙雷氏菌 alkaline protease catabolite repression Serratia marcescens Q556.3;Q591.9 1993-01-01 2021-04-01 1