耐甲氧西林金黄色葡萄球菌对多种中药制剂敏感性研究

耐甲氧西林金黄色葡萄球菌对多种中药制剂敏感性研究 Sensitivity of the Methicillin-Resistant Staphylococcus aureus Strains to Several Traditional Chinese Medicine Preparations 陈林娜 CHEN Lin-na 1 2 first-author 李波 LI Bo 1 2 刘玲 LIU Ling 1 2 周立勤 ZHOU Li-qin 1 2 王春香 WANG Chun-xiang 1 2 王汉敏 WANG Han-min 1 2 谭大奇 TAN Da-qi 1 2 湖北中医学院附属医院 湖北中医学院附属医院 Hospital Affiliated to the Hubei College of Traditional Chinese Medicine Hospital Affiliated to the Hubei College of Traditional Chinese Medicine 湖北中医学院附属医院,湖北,武汉,430061 湖北中医学院附属医院,湖北,武汉,430061 采用血清药理学检测方法,用不同含药血清最低抑菌滴度进行耐药菌株的抑菌实验,了解甲氧西林耐药金黄色葡萄球菌（MRSA）对热必宁等3种中药制剂的敏感性,并用透射电镜观察含药血清作用后实验菌菌体形态变化.含药血清对临床分离的MRSA菌株的抑菌试验表明:3种中药制剂抑菌效果优于阴性组,有极显著意义（P<0.01）,其中肺炎一号、清胆糖浆作用更为显现,抑菌效果优于阳性组.同时电镜微细结构观察亦可证实中药制剂破坏了细菌胞壁结构,细菌形态结构发生改变.结果显示,中药制剂对MRSA临床分离株有明显抑菌作用. Refering to the theory of the serum pharmacology,inhibitory testings to test minior inhibitory titers could be done with the medicine-containing sera of different concentrations and resistant strains.So the medicine-containing sera and the methicillin-resistant Staphylococcus aureus（MRSA） clinical strains were used to do inhibitory testings,the electron microscope to observe the changes taken place on the tested cells.The results showed that the inhibitory effects of three preparations were superior to those of the negative group（P<0.01）.Additionally,The inhibitory effects of the preparations such as Pneumomiae No.1 and Qingdan Syrup were also superior to those of the positive group（P<0.01）.Meanwhile,the destroyed cell walls of the tested MRSA strains were observed,as well as other cell structures.In conclusion,the clinical MRSA strains could be inhibited obviously by the preparations. 血清药理学 中药含药血清 耐甲氧西林金黄色葡萄球菌 抑菌试验 serum pharmacology serum containing medicine methicillin-resistant Staphylococcus aureus inhibitory testings R285.5 湖北省教育厅资助项目（2001A04009） 2005-01-01 2021-04-01 S2