羽衣甘蓝组织培养研究

羽衣甘蓝组织培养研究 Tissue Culture of Ornamental Kale 孟志卿 MENG Zhi-qing 1 2 first-author 徐东生 XU Dong-sheng 1 2 王继珍 WANG Ji-zhen 1 2 孝感学院生命科学技术学院 孝感学院生命科学技术学院 College of Lifes and Technology,Xiaogan College College of Lifes and Technology,Xiaogan College 孝感学院生命科学技术学院,湖北,孝感,432100 孝感学院生命科学技术学院,湖北,孝感,432100 以羽衣甘蓝的花蕾、花茎和腋芽为外植体,进行组织培养试验.结果表明:花蕾、花茎消毒时间以5 7 min为好,腋芽消毒时间以8 min为宜,以腋芽为外植体,以MS基础培养+6-苄基腺嘌呤（BA）2 mg+萘乙酸（NAA）0.2 mg+3%蔗糖进行培养较好,继代培养以MS基础培养+BA 3 mg+NAA 0.2 mg+3%蔗糖增殖率最高;在生根阶段,以50%MS（1 L）+NAA0.4 mg+1%蔗糖生根效果最好,生根率达94.79%;移栽基质以蛭石最好,羽衣甘蓝组培苗成活率达86.7%,且生长健壮. The flower buds,pedicels and axillary of ornamental kale were cultured in vitro.The optimum disinfection time was 5 7 minutes for flower buds and pedicels,and was 8 minutes for axillary.MS+2 mg/L BA + 0.2 mg/L NAA+3% cane sugar was optimum medium for induction of axillary,MS+3 mg/L BA+0.2 mg/L NAA+3% cane sugar was the best medium for its proliferation,and 50%MS +0.4 mg/L NAA +1% cane sugar was the best medium for its rooting and the rooting rate was 94.8%.Vermiculite was the optimum matrix for its transplant,and the survival rate was 86.7%. 羽衣甘蓝 外植体 组织培养 ornamental kale explants tissue culturing S635.9;S681.9 2005-01-01 2021-04-01 S2