苏云金芽胞杆菌的分离与鉴定及高毒力菌株的筛选

苏云金芽胞杆菌的分离与鉴定及高毒力菌株的筛选 Isolation and Identification of Bacillus thuringiensis 彭可凡 PENG Ke-fan 1 2 first-author 戴顺英 DAI Shun-ying 1 2 陈讯 CHEN Xun 1 2 高梅影 GAO Mei-ying 1 2 中国科学院武汉病毒研究所 中国科学院武汉病毒研究所 Wuhan Institute of Virology,Chinese Academy of Sciences Wuhan Institute of Virology,Chinese Academy of Sciences 中国科学院武汉病毒研究所,湖北,武汉,430071 中国科学院武汉病毒研究所,湖北,武汉,430071 从湖北、湖南等省的土壤中分离得到14株苏云金芽胞杆菌菌株.血清型分析表明,苏云金芽胞杆菌分离株分别属于30个血清型中的3个血清型,另有两个与所用标准菌株的抗血清无凝集反应的菌株.观察和测定了苏云金芽胞杆菌分离株的形态和毒力,并用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析了菌株的晶体蛋白成分,筛选到2株高效杀鳞翅目害虫的苏云金芽胞杆菌;并用特异性引物PCR检测了这些菌株的杀虫基因. Some isolates of Bacillus thuringiensis（Bt） were obtained from soils of Anhui and Hubei Provinces of China.Most Bt isolates belonged to 3 sorts of 30 serotypes,and 2 of them had no reaction with all standard serotypes.The crystal proteins of Bt isolates were investigated.2 of Isolates were high activity against Lepidopteron.The cry gene had been tested by PCR.It was shown that Bacillus thuringiensis is actually soil microorganism. 苏云金芽胞杆菌 鞭毛血清型 SDS-PAGE电泳 Bacillus thuringiensis serotype SDS-PAGE S476.11 国家自然科学基金资助项目（30170025,30370778） 2005-01-01 2021-04-01 S2