对虾白斑综合征病毒VP28免疫后克氏原螯虾血清酶活性及中和活性

对虾白斑综合征病毒VP28免疫后克氏原螯虾血清酶活性及中和活性 Lysozyme,Phenoloxidase Activity and Neutralization Ability in the Serum of Crayfish Vaccined by VP28 of White Spot Syndrome Virus 袁军法 YUAN Jun-fa 1 2 first-author 李莉娟 LI Li-juan 1 2 谷万港 GU Wan-gang 1 2 祝璟琳 ZHU Jing-lin 1 2 石正丽 SHI Zheng-li 1 2 中国科学院武汉病毒研究所/病毒学国家重点实验室 中国科学院武汉病毒研究所/病毒学国家重点实验室 Wuhan Institute of Virology,Chinese Academy of Sciences/State Key Laboratory of Virology Wuhan Institute of Virology,Chinese Academy of Sciences/State Key Laboratory of Virology 中国科学院武汉病毒研究所/病毒学国家重点实验室,湖北,武汉,430071 中国科学院武汉病毒研究所/病毒学国家重点实验室,湖北,武汉,430071 利用原核表达的对虾白斑综合征病毒（white spot syndrome virus,WSSV）结构蛋白VP28口服免疫克氏原螯虾,以酚氧化酶和溶菌酶指标来监测机体的免疫反应,并检测免疫后的螯虾血清对WSSV的中和能力.结果表明VP28能有效激起机体的免疫反应,酚氧化酶活性始终处于较低的水平,低于GST对照组和健康组,溶菌酶活性则高于GST对照组和健康组.中和实验表明免疫血清具有一定的中和活性,但其保护作用不是通过VP28进入体内与细胞受体结合,来阻止病毒进入,而可能是通过病毒蛋白进入虾体后刺激机体产生或分泌某种中和因子,从而阻止病毒进入细胞或进入细胞后阻止进一步传播. White spot syndrome virus（WSSV） is currently the most serious virus pathogen of shrimp culture,the development of vaccines against WSSV would be desirable.To study the mechanism of immune response induced by one WSSV envelope protein,VP28,the crayfish procambarus clakii was fed with food pellets coated with inactivated bacteria overexpressing VP28.The activity of lysozyme and phenoloxidase in serum of crayfish postvaccination was detected.And the neutralization activity of the serum was investigated.The results showed VP28 could induce the immune response and protection against WSSV infection to crayfish.The activity of phenoloxidase was lower than that of glutathione S-lransfarase（GST） and negative control group,while the activity of lysozyme was higher than that of GST and negative control group.The results of neutralization experiment showed that the serum of crayfish postvaccination provided protection,which might not by blocking with cellar acceptors needed by the virus to enter cells,but by stimulating the shrimp to excrete neutralizing substances or by blocking virus spread after entry. 克氏原螯虾 对虾白斑综合征病毒 囊膜蛋白 中和 crayfish white spot syndrome virus envelope protein neutralization S945.4 国家高技术研究发展计划（863）资助项目（2003AA620201）;中国科学院生命科学与生物技术领域2001年知识创新工程重要方向项目（KSCX2-SW-302） 2005-01-01 2021-04-01 S2