合浦珠母贝受精细胞学观察

合浦珠母贝受精细胞学观察 CYTOL OGICAL OBSERVATIONS ON MEIOSIS AND FERTILIZATION OF PEARL OYSTER PINCTADA MARTENSII DUNKER 沈亦平 Shen Yiping Liu Ting Jiang Haibo Ye Li Zhang Xiyuan 1 first-author 刘汀 1 姜海波 1 叶力 1 张锡元 1 武汉大学生命科学学院 武汉大学生命科学学院 School of life Science, Wuhan University School of life Science, Wuhan University 用醋酸——地衣红染色制片的方法对台浦珠母贝卵子减数分裂和受精过程进行了详细的细胞学观察.精子入卵前,卵子停留于第一次减数分裂中期.精子入卵后,卵子开始成熟分裂,在海水温度22℃的条件下,授精后22 min 及39 min 绝大部分卵子分别完成第一次减数分裂和第二次减数分裂,排出第一极体和第二极体,雌雄原核分别形成,其染色体逐渐凝集,形成两组染色体,最后在卵裂的赤道板上联合,71 min 后,大部分卵子完成第一次卵裂.卵子发育的非同步性现象非常明显.我们还观察到部分卵子在尚未有精子入卵的情况下即开始进行减数分裂,而且能够继续发育,暗示合浦珠母贝卵子具有雌核发育的可能. The events of meiosis and fertilization in pearl oyster, Pinctada martensii Dunker were in- vestigated with aceto——orcein stain method. Eggs were fertilized at 22℃, before sperm penetration, all eggs were at metaphase of first meiosis. Eggs completed first and second meiosis by 22min and 39 rain after insemination respectively. The chromatin of male and femaie pronuclear condensed separate- ly and they combined finally on their metaphase plate of first cleavage. By 71 rain most eggs complet- ed first cleavage. Asynchronous development of eggs were observed. The observation also imply the" gynogenesis" of oyster eggs. 合浦珠母贝 减数分裂 受精 非同步性发育 Pinctada martensü Dunker meiosis fertilization synchronous development Q953.1;Q954.44 国家自然科学基金(61672102);广西壮族自治区地方重大项目资助的课题 1993-05-01 2021-04-01 5