合浦珠母贝卵子成熟的细胞学观察

合浦珠母贝卵子成熟的细胞学观察 CYTOLOGY OF EGG MATURATION OF PEARL OYSTER PINCTADA MARTENSII DUNKER 沈亦平 Shen Yiping Jiang Haibo Liu Ting Zhang Xiyang 1 first-author 姜海波 1 刘汀 1 张锡元 1 武汉大学生命科学学院 武汉大学生命科学学院 School of Life Science, Wuhan University School of Life Science, Wuhan University 用细胞学的方法对合浦珠母贝卵子在体内和体外的成熟过程进行了观察,结果发现:性腺内发育的卵子大多停留于第一次减数分裂的前期,处于排卵状态时,性腺内卵子进一步发育至第一次减数分裂的前中期,解剖性腺取出的卵子能在海水中继续发育达到第一次减数分裂中期,氨海水能够促进这一发育过程.自然排放的卵子在刚刚接触到海水时仍处于第一次减数分裂的前中期,它们的进一步成熟,即发育至第一次减数分裂中期是在海水中完成的.文中对合浦珠母贝卵子成熟度的检测进行了讨论. Maturation of pearl oyster eggs both in vivo and in vitro was examined with aceto--or- cein stain method. We found out that: （1）. Eggs in ovaries are usually restricted to meiotic prophase, they can undergo germinal vesicle breakdown when ready for spawn hut pause at prometaphase. （2）, Dissected eggs contain germinal vesicles’and when placed in seawater, they undergo germinal vesicle brackdown adn mature to the first metaphase of meiosis. Alkaline seawater （0. 0050 ammonia in sea- water）can promote this process. （3）. Eggs just after spawning are at prometaphase ,they soon mature to metaphase in seawater and then ready for fertilization. 合浦珠母贝 卵子发育 细胞生物学 Pinctada martensii Dunker maturation of eggs development of eggs in vivo and in vitro Q954.432;Q953.1 国家自然科学基金(61672102);广西壮族自治区地方重大项目资助的课题 1993-05-01 2021-04-01 5