羊毛作载体吸附固定地衣芽孢杆菌产碱性蛋白酶的研究

羊毛作载体吸附固定地衣芽孢杆菌产碱性蛋白酶的研究 STUDY ON IMMOBILIZED BACILLUS LICHENIFORMIS BY SHEEPWOOL ABSORBING PRODUCING ALKALINE PROTEASE 邓晓皋 Deng Xiaogao 1 2 3 first-author 彭珍荣 Peng Zhenrong 1 2 3 武汉大学生物学系 武汉大学生物学系 Department of Biology, Wahan university Department of Biology, Wahan university 武汉大学生物学系,武汉 武汉大学生物学系,武汉 ,430072 ,430072 在菌悬液浓度为2.5×10 10 个·mL -1 ,离子强度0.15mol·L -1 。NaCl,pH6.5平衡吸附5h的条件下,每克羊毛可吸附0.43g湿重细胞,此固定化细胞在合成培养基上发酵42h达产酶高峰,比游离细胞的发酵周期缩短18h,其摇瓶半连续发酵比游离细胞分批发酵产酶的效率高43%,而发酵液中菌浓度低10～100倍,固定化细胞已重复使用12次。 Under the condition as following: 0.15mol·L -1 NaCl, 2.5×10 10 cells·mL -1 , pH6.5 and 5 hours’ absorbing, a gram sheep wool can absorb 0.43 gram wet cells. When the immobilized cells were cultured in the synthesis medium,enzyme activity was the highest after 42 hours. By comparing portease production of immobilized cell with free cell, the result showed that the fermentation cycle of the former was 18 hours shorter than the latter) and the bacterial concentration in the former fermentation medium was 10～100 times lower. Immobilized cell with semicontinuous fermentation was used 12 times, and the fermentation efficiency of immobilized cells was 43 percent higher than that of free cells in batch fermentation. 羊毛 吸附固定化 碱性蛋白酶 sheep wool absorbent immobilized alkaline protease Q936 1993-02-01 2021-04-01 2