邮递和保存质粒DNA的简易有效方法

邮递和保存质粒DNA的简易有效方法 A SIMPLE AND EFFICIENT METHOD FOR PLASMID DNA SHIPMENT AND STOPAGE 程汉华 1 first-author 周荣家 1 武汉大学生命科学学院 武汉大学生命科学学院 School of Life Sciences , Wuhan University School of Life Sciences , Wuhan University 邮递和保存质粒ＤＮＡ的简易有效方法程汉华，周荣家（武汉大学生命科学学院，武汉，４３００７２）中国法分类号Ｑ７８１随着现代分子生物学研究工作的不断深入和发展，国家或地区间的科技交流活动日趋平凡．常常需要在不同国家或地区的实验室之间邮递或携带质粒ＤＮＡ．... Plasmid DNA solution was doted onto a piece of Whatman 3MM paper and placed intoa sealable bag and heat sealed. Plasmid could be sent then by mail or stored at 4℃. For plasmid recovery, cut the circle out and rehydrate. The supernatant liquid was used to transform competent bacteria.For verification,the plasmid DNA was isolated and cut by restriction enzymes and amplified by polymerase chain reaction. Q781 1994-02-01 2021-04-01 2