利用模拟代谢活化体系研究污染物的基因毒性

利用模拟代谢活化体系研究污染物的基因毒性 Gene Toxicity of Pollutants by Utilizing the Chemical Model of Metabolic Activation System 吴新国 WU Xin-guo 1 2 first-author 严炜 YAN Wei 1 2 徐靖 XU Jing 1 2 武汉大学资源与环境科学学院 武汉大学资源与环境科学学院 College of Resources and Environmental Sciences,Wuhan University College of Resources and Environmental Sciences,Wuhan University 武汉大学资源与环境科学学院,湖北,武汉,430079 武汉大学资源与环境科学学院,湖北,武汉,430079 利用二氯乙烷、三氯乙烷、三氯乙烯、N,N-二甲基甲酰胺、丙酮作为典型污染物与Fe2+/EDTA/H2O2/联氨模拟体系反应,所产生的活性中间体与4-（对硝基苄基）吡啶反应生成了紫红色物质.据此,本文建立了一种分光光度法检测活性中间产物的新方法.该方法测量灵敏度高（10-6mol/L）.应用该方法证实了其中4种污染物（二氯乙烷、三氯乙烷、三氯乙烯、N,N-二甲基甲酰胺）具有形成DNA加合物的能力.同时对代谢活化体系的各种显色条件进行了优化. As typical pollutants,dichloroethane,trichloromethane,trichloroethylene,N,N-Dimethylformamide and acetone were selected to react with the mimic system of Fe 2+ /EDTA/H 2 O 2 /hydrazine,and the reactive intermediates formed.Further reaction of the reactive intermediates and 4-（4-nitrobenzyl）pyridine gave a red-purple colored substance.Based on this,a new method for the detecting of the reactive intermediates was established with a high sensitivity of 10 -6 mol/L.Through this method,it is verified that dichloroethane,trichloromethane,trichloroethylene,N,N-dimethylformamide have the ability to form DNA adducts.A variety of colouring conditions for the metabolic activation system were also optimized. 代谢活化 化学模拟 4-硝基苄基吡啶 metabolic activation chemical model 4-（4-nitrobenzyl）pyridine X171 国家自然科学基金资助项目（20377032） 2005-06-01 2021-04-01 6