泽苔草居群内遗传多样性

泽苔草居群内遗传多样性 Genetic Diversity in a Natural Caldesia parnassifolia Population 董贞荣 DONG Zhen-rong 1 2 first-author 陈进明 CHEN Jin-ming 1 2 王青锋 WANG Qing-feng 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 采用RAPD分子标记对泽苔草湖里居群10个家系共60个子代样品进行了家系间及家系内的遗传多样性分析.12个有效引物共产生91条带,其中83条带表现出多态性,占91.21%.各个家系的基因多样性在0.11 0 .23之间（家系水平为0.27）,Shannon指数在0.17 0 .34之间（家系水平为0.41）.分子变异分析（AMOVA）结果显示家系间的基因分化系数（Gst）为0.277,说明家系间的遗传变异占27.7%,家系内的遗传变异占72.31%,家系间和家系内的变异均极显著（p < 0.001）.基于家系间的遗传分化系数（Gst）估测家系间的基因流（Nm）为0.65.采用UPGMA法对所有子代聚类结果表明:同一家系的子代个体并不能完全聚在一起. Genetic diversity in a natural Caldesia parnassifolia population in Hunan Province was assessed using RAPD markers.A total of 60 individuals from 10 maternal plants were analyzed.Using twelve primers,which produced highly reproducible bands,a total of 91 DNA fragments were generated with 83（91.21%） being polymorphic.Analysis of molecular variance（AMOVA） showed that a large proportion of genetic variation（72.31%） resided within maternal plants,while only a small proportion（（27.69%）） resided among maternal plants.Shannon index showed that the total genetic diversity among maternal plants was 0.4147,which was identical with the result of Nei’s gene diversity.NTSYS analysis indicated that the offspring individuals from a maternal plant could not be clustered completely together.The estimate of gene flow（N m ） among maternal plants was 0.6528. 濒危植物 泽苔草 家系 分子变异分析 随机扩增多态性 endangered plant Caldesia parnassifolia family analysis of molecular variance（AMOVA） random amplified polymorphic DNA（RAPD） Q943 国家重点基础研究发展计划（973）资助项目（G2000046805）;国家自然科学基金资助项目（30370098） 2005-06-01 2021-04-01 6