乙醇对颈上神经节神经元钙内流的影响

乙醇对颈上神经节神经元钙内流的影响 Effect of Voltage-Dependent Calcium Channels and Ionophore Channels on Cultured Superior Cervical Ganglion by Ethanol 肖哲曼 XIAO Zhe-man~1 1 first-author 卢祖能 LU Zu-neng~1 1 郑建全 ZHENG Jian-quan~2 2 余绍祖 YU Shao-zu~1 1 李立君 LI Li-jun~2 2 张树卓 ZHANG Shu-zhuo~2 2 武汉大学人民医院神经内科 武汉大学人民医院神经内科 Department of Neurology,Renmin Hospital of Wuhan University Department of Neurology,Renmin Hospital of Wuhan University 军事医学科学院毒物药物研究所 军事医学科学院毒物药物研究所 Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences 分散、培养新生12 h内大鼠的颈上交感神经节细胞,应用全细胞膜片钳和共聚焦显微技术观察不同浓度乙醇对颈上神经节细胞钙内流的作用.10,30,100,300,600 mmol/L乙醇对钙电流峰值的抑制率依次为0.080±0.031,0.120±0.053,0.277±0.062,0.570±0.082和0.895±0.054.100 mmol/L乙醇可将40 mmol/L KCl诱发的胞浆内钙离子浓度（[Ca2+]i）增高从1.725±0.159显著抑制至1.156±0.135,可将1μmol/L钙离子载体A23187诱发的[Ca2+]i增高从2.232±0.015显著抑制至1.263±0.047.故乙醇对交感神经元细胞外钙内流具抑制作用,急性乙醇中毒可能与其抑制钙电流有一定关系. The effect of ethanol on voltage-dependent calcium channel in the cultured superior cervical ganglion was observed with conventional whole-cell patch clamp technique.Cytoplasmic calcium concentration（-i） changes caused by ethanol were measured by monitoring the alteration of cell fluorescence.Inhibition rate of calcium current by 10,30,100,300,600 mmol/L ethanol was 0.080±0.031,0.120±（0.053,）0.277±0.062,0.57±0.082 and 0.895±0.054.But ethanol did not affect the decay of calcium current,the voltage dependence or the time course of activation.100 mmol/L ethanol significantly inhibited -（i） increments evoked by 40 mmol/L KCl from 1.725±0.159 to 1.156±0.135（F/F 0 ）.Inhibition of 1 μmol/L A23187-induced -i increment by 100 mmol/L ethanol was significant from 2.232±0.015 to 1.263±0.047（F/F 0 ）.Ethanol inhibits the calcium influx through VDCCs and ionophore channels significantly and dosage dependently. 乙醇 电压门控钙通道 颈上神经节 膜片钳 共聚焦 ethanol voltage-dependent calcium channel superior cervical ganglion patch clamp confocal R363 湖北省科技厅基金项目（2001AA307B18） 2005-06-01 2021-04-01 6