青木香挥发油的化学成分分析及抗菌活性

青木香挥发油的化学成分分析及抗菌活性 Chemical Composition and Antimicrobial Activity of the Essential Oil of Aristolochia debilis sieb.et Zucc. 朱顺英 ZHU Shun-ying 1 2 first-author 杨扬 YANG Yang 1 2 侯洁 HOU Jie 1 2 于怀东 YU Huai-dong 1 2 邹国林 ZOU Guo-lin 1 2 武汉大学生命科学学院/病毒学国家重点实验室 武汉大学生命科学学院/病毒学国家重点实验室 College of Life Sciences,Wuhan University/State Key Laboratory of Virology College of Life Sciences,Wuhan University/State Key Laboratory of Virology 武汉大学生命科学学院/病毒学国家重点实验室,湖北,武汉,430072 武汉大学生命科学学院/病毒学国家重点实验室,湖北,武汉,430072 采用GC-MS技术利用3种不同极性的毛细管柱分析了青木香挥发油的化学组成,确定了26种成分的化学结构与相对含量,结果表明其挥发油主要由单萜组成,其中莰烯（23.98%）、异甲酸龙脑脂（19.40%）、Selina-1,3,7（11）-trien-8-one（10.24%）和龙脑（6.82%）是主要成分.对3种真菌和1 3种细菌（包括7种临床致病菌）的药敏实验表明,挥发油对大部分微生物均具有很好的抗菌作用,尤其对革兰氏阳性菌的抗菌活性更强,其最低抑菌浓度（MIC）为0.04 0 .31 g/L,最低杀菌浓度（MBC）为0.08 2 .5 g/L. Essential oil of subterranean part of Aristolochia debilis sieb.et Zucc.was analyzed by GC-MS in three different polarities.The results show that twenty-six components were identified in the oil,and main compounds of both oil were camphene（23.98%）,isobornyl formate（19.40%）,selina-1,3,7（11）-trien-8-one（10.24%） and borneol（6.82%）.The antimicrobial activity of the oil was evaluated （against） 3 fungi and 13 bacteria including 7 clinical isolated strains.Our results showed that the oil presented a broad antimicrobial spectrum,and had better antimicrobial activity against Gram-positive bacteria,the MIC values were 0.04～0.31 g/L and the MBC values was 0.08～2.5 g/L. 青木香 挥发油 抗菌 气相色谱-质谱法 Aristolochia debilis essential oil antimicrobial activity GC-MS R284;R285 2005-06-01 2021-04-01 6