外源Ca²⁺参与铅对盐藻毒害作用的研究

外源Ca 2+ 参与铅对盐藻毒害作用的研究 Effect of Extracellular Calcium on the Toxicity of Lead to Dunaliela salina 孟祥红 MENG Xiang-hong 1 2 first-author 刘成圣 LIU Cheng-sheng 1 2 于乐军 YU Le-jun 1 2 周丽 ZHOU Li 1 2 陈西广 CHEN Xi-guang 1 2 中国海洋大学海洋生命学院 中国海洋大学海洋生命学院 College of Marine Life Sciences, Ocean University of China College of Marine Life Sciences, Ocean University of China 中国海洋大学海洋生命学院,山东,青岛,266003 中国海洋大学海洋生命学院,山东,青岛,266003 研究了不同浓度Pb2+对盐藻生长的胁迫作用及外源10mmol/LCa2+、La3+对Pb2+胁迫的影响.Pb2+抑制盐藻的生长,其抑制程度与Pb2+浓度成正相关;外源Ca2+在初期可缓解Pb2+对盐藻生长的抑制作用,后期加重Pb2+对盐藻生长的抑制;La3+处理加重Pb2+对盐藻生长的胁迫作用.实验结果初步表明外源Ca2+内流参与盐藻对Pb2+胁迫的适应性调节作用. The effects of lead stress at various concentration of （12 μg/L, 50 μg/L,0.1 mg/L, 0.5 mg/L,1.0 mg/L） on growth of Dunaliela salina, and the roles of Ca 2+ and La 3+ under lead stress were investigated, respectively. The results show that growth of Dunaliela salina was inhibited by lead with varieties concentration, and there was a positive correlation between inhibitory effect and lead concentration （from 12 μg/L to 1.0 mg/L）, while participation of extracellular Ca 2+ （10 mmol/L）, inhibitory effect of lead to growth of Dunaliela salina was weakened at preliminary stage, but strengthened at late stage, separately, other than Ca 2+ , due to participation of La 3+ （10 mmol/L）, the inhibitory effect of lead was throughout intensified at various concentration. In conclusion, the results indicate that extracellular calcium played an important adjusting role under lead stress to Dunaliela salina. 盐藻 铅离子胁迫 Ca2+ La3+ Dunaliela salina lead stress Ca2+ La3+ Q948.116 2005-04-01 2021-04-01 4