四羧基锰（Ⅲ）酞菁与ONOO^-的相互作用

四羧基锰（Ⅲ）酞菁与ONOO - 的相互作用 Interaction Between Mn（Ⅲ）TcPc and ONOO - 陈东明 CHEN Dong-ming~ 1 2 3 4 first-author 潘逆娜 1 2 3 4 骆华 1 2 3 4 李得加 1 2 3 4 邹国林 1 2 3 4 武汉大学生命科学学院 武汉大学生命科学学院 1,2),PAN Ni-na~1, LUO Hua~1,LI De-jia~1, ZOU Guo-lin~1 1,2),PAN Ni-na~1, LUO Hua~1,LI De-jia~1, ZOU Guo-lin~1 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 怀化学院生物系 怀化学院生物系 ,湖北,武汉,430072 ,湖北,武汉,430072 研究了四羧基锰（Ⅲ）酞菁（Mn（Ⅲ）TcPc）与ONOO-的相互作用及其对ONOO-硝基化酪氨酸（Tyr）和损伤DNA的影响.结果表明Mn（Ⅲ）TcPc能与ONOO-作用,并可促进ONOO-对Tyr的硝基化,该硝基化作用能被谷胱甘肽（GSH）或抗坏血酸（VitC）等生物还原剂抑制;该硝基化作用与pH值有关,以pH7为最合适.Mn（Ⅲ）TcPc能抑制ONOO-对DNA的损伤作用.GSH对Mn（Ⅲ）TcPc有还原作用.并对上述的促进或抑制Tyr硝基化反应的机理进行了理论解释. Peroxynitrite（ONOO - ） have strong oxidation property implicated in many biological conditions. So we have explored the interaction between manganese（Ⅲ） tetracarboxylphthalocyanine\ and ONOO - . Mn（Ⅲ）TcPc was characterized with respect to ONOO - decomposing activities, but enhanced the yield of nitrated phenolics by ONOO - . Nitration enhancement by Mn（Ⅲ）TcPc was effectively inhibited by glutathione（GSH） or ascorbate（Vit C）, although much higher concentrations of Vit C were required to inhibit nitration catalyzed by Mn（Ⅲ）TcPc. It is reasonable to suggest that Mn（Ⅲ）TcPc could detoxify ONOO - in vivo by efficiently trapping the relatively unreactive ONOO - and, in effect, channeling it into a single reaction pathway which could then be more effectively scavenged by cellular reductants like GSH or Vit C. 四羧基锰（Ⅲ）酞菁 过氧亚硝基阴离子 酪氨酸硝基化 manganese（Ⅲ）tetracarboxylphthalocyanine peroxynitrite tyrosinenitration R363 国家自然科学基金资助项目（30370366） 2005-04-01 2021-04-01 4