光敏感核不育水稻愈伤组织的超低温保存和冻后再生植株的形成

光敏感核不育水稻愈伤组织的超低温保存和冻后再生植株的形成 CRYOPRESERVATION AND REGENERATION OF CALLUS OF PHOTOPERIOD SENSITIVE GENIC MALE STERILE RICE 田永中 1 2 3 first-author 舒理慧 1 2 3 郑从义 1 2 3 武汉大学生命科学学院 武汉大学生命科学学院 School of life Sciences,Wuhan University School of life Sciences,Wuhan University 珠海丽珠制药厂 珠海丽珠制药厂 武汉大学生命科学学院珠海丽珠制药厂 武汉大学生命科学学院珠海丽珠制药厂 研究了光敏感核不育水稻的悬浮细胞及愈伤组织的低温贮藏方法．实验采用１０％ＤＭＳＯ＋０．５ｍｏｌ·Ｌ－１山梨醇作冰冻保护剂，降温速率采用１．０℃·ｍｉｎ－１或０．５℃·ｍｉｎ－１，ＴＴＣ法测试的细胞存活率最高可达８５．９％，冻后材料经１５ｄ左右即可在暗培养条件下观察恢复生长的情况．３０ｄ后把长势良好的愈伤组织块转移至分化培养基中，来源于悬浮细胞团的绿苗分化率为１０．０％，愈伤组织的则为６６．６％． This paper mainly reported the cryopreservation method of callus and cellsuspensions of HPGMR. Cell line （N5047s）and callus （Nong Keng 58s） were derived fromyoung panicles culture. By preculturing-freezing-liquid nitrogen cryopreserving-rapidthawing, the highest cell viability （TTC test）was 85. 9%.The mixture of cryoprotectant of 10%DMSO plus 0. 5ml0. 5L-1 sorbitol was optimal,and slow freezing with a temperature of 1.0℃· min-1 or 0. 5℃·min-1 was recommended. After 15 days’ dark culture, the recoverygrowth of frozen materials was observed. Then we transfered the callus to differentiation culture medium. The plantlet differentiation rate from cell suspensions was 10. 0%, and thatfrom callus was 66. 6%. 光敏感核不育水稻 悬浮细胞 愈伤组织 超低温保存 植株再生 HPS GMR （rice） cell suspensions callus cryopreservation plant regeneration S511.032 国家自然科学基金(61672102) 1994-04-01 2021-04-01 4