正丁醇中氯化血红素催化邻苯二胺氧化反应中间体

正丁醇中氯化血红素催化邻苯二胺氧化反应中间体 Intermediate in the o-Phenylenediamine Oxidation Reaction Catalyzed by Hemin in n-Butanol 李得加 LI De-jia 1 2 first-author 彭小伟 PENG Xiao-wei 1 2 李希文 LI Xi-wen 1 2 邹国林 ZOU Guo-lin 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 比较了在不同有机溶剂中,以氯化血红素模拟辣根过氧化物酶催化过氧化氢氧化邻苯二胺的反应,发现在正丁醇中氯化血红素具有明显的过氧化物酶活性,能催化过氧化氢氧化邻苯二胺的反应,并可产生稳定的中间体.研究了中间体形成的动力学和不同温度、pH值和溶剂对中间体稳定性的影响.结果表明,中间体的半衰期在pH 7.5左右最大;在25 ℃时,中间体在正丁醇中的存在时间是在水中的3倍. The oxidative reaction of o-phenylenediamine with hydrogen peroxide catalyzed by hemin was detected in n-butanol. The characteristics of the reaction intermediate were studied. The experiment results show that hemin can catalyze the reaction of o-phenylenediamine with H 2 O 2 , and the intermediate stability of the reaction is variable as temperature and pH changed. The intermediate had the longest half life time at pH 7.5 and its exist time in n-butanol is three times as long as that in water at 25℃. 氯化血红素 模拟酶 中间体 正丁醇 Hemin mimetic enzyme intermediate n-butanol O621.251 国家自然科学基金资助项目（39770200） 2005-02-01 2021-04-01 2