耐亚胺培南铜绿假单胞菌整合子结构分析

耐亚胺培南铜绿假单胞菌整合子结构分析 Structure of a Class I Integron Harbered in a Clinical Isolate of Pseudomonas aeruginosa 彭少华 PENG Shao-hua 1 2 first-author 顾剑 GU Jian 1 2 刘素玲 LIU Su-ling 1 2 程文娟 CHENG Wen-juan 1 2 李从荣 LI Cong-rong 1 2 武汉大学人民医院 武汉大学人民医院 Renmin Hospital, Wuhan University Renmin Hospital, Wuhan University 武汉大学人民医院,湖北,武汉,430060 武汉大学人民医院,湖北,武汉,430060 运用PCR mapping、DNA测序技术和生物信息学手段,对一株临床分离耐亚胺培南铜绿假单胞菌进行整合子结构分析.结果发现了一个新的多重耐药I型整合子,它含有一个blaVIM 2 基因盒（编码金属β内酰胺酶）和一个新的aacA4样基因盒（编码6′N 氨基糖苷乙酰转移酶）,并具有一个强的整合酶结合位点.新的多重耐药整合子的发现,表明了整合子基因盒系统的高度异质性和铜绿假单胞菌多重耐药机制的复杂性. With PCR-mapping, DNA sequencing and bioinformatics approach, structure of an integron in a clinical isolate of Pseudomonas aeruginosa resisting to imipenem was studied. As result, a new integron with multi-resistance potential was identified. It contains a bla VIM-2 gene cassette, which encoding a metallo-beta-lactamase, and an aacA4-like gene cassette, which encoding aminoglycoside 6′-N-acetyltransferase. Furthermore, the integron has a strong integrase binding site. All these imply the heterogeneity of intergron-gene cassette system in bacterial and the complexity of drug resistance mechanism in Pseudomonas aeruginosa. 铜绿假单胞菌 耐药 整合子 亚胺培南 金属β内酰胺酶 Pseudomonas aeruginosa drug resistance integron imipenem metallo-beta-lactamase R446.5 湖北省卫生厅重点资助项目（WJ01564） 2005-02-01 2021-04-01 2