琼脂糖蛋白质A反向免疫共沉淀法纯化蓝舌病毒HbC₃

琼脂糖蛋白质A反向免疫共沉淀法纯化蓝舌病毒HbC 3 Purifying BTV-HbC 3 by Agarose Protein a Reverse Co-Immunoprecipitation 刘文培 LIU Wen-pei 1 2 first-author 董长垣 DONG Chang-yuan 1 2 刘文惠 LIU Wen-hui 1 2 郭淑芳 GUO Shu-fang 1 2 卢莉莉 LU Li-li 1 2 王海琪 WANG Hai-qi 1 2 武汉大学医学院/病毒学国家重点实验室 武汉大学医学院/病毒学国家重点实验室 School of Medicine, Wuhan University/State Key Labratory of Virology School of Medicine, Wuhan University/State Key Labratory of Virology 武汉大学医学院/病毒学国家重点实验室,湖北,武汉,430071 武汉大学医学院/病毒学国家重点实验室,湖北,武汉,430071 用高效液相色谱（HPLC）、透射电镜检测琼脂糖蛋白质A反向免疫共沉淀法纯化蓝舌病毒湖北株（BTV HbC3）的效果,组织培养半数感染量（TCID50）比较其纯化前后的生物学活性.结果显示,纯化过程对病毒生物学活性无明显影响,透射电镜下,纯化病毒背景清晰, HPLC解析出纯化物仅单一病毒峰.本纯化体系用于提纯BTV HbC3 效果显著,操作简便,具备高通量高活性纯化病毒的潜能,为建立大量纯制高活性蓝舌病毒用于实验室靶向性抗癌研究和生产实践奠定技术平台. Using HPLC and electron microscopic to detect BTV-HbC 3 purified by agarose Protein A reverse co-immunoprecipitation. The results showed that the background of the purifying virus is clean and clear. HPLC showed that the compound of the purifying virus is a single peak. TCID 50 revealed that the viral infectivity is maintained. The conclusion is that this method is feasible, simple and holds the potential of high throughput of purifying virus. This is a base for producing high yield and high infectivity bluetongue virus as a nature oncolytic virus. 病毒纯化 反向免疫共沉淀 蓝舌病毒 virus purification reverse co-immunoprecipition bluetongue virus S852.65 国家自然科学基金资助项目 （30271471） 2005-02-01 2021-04-01 2