黄鳝DEAD-box家族PL10基因的克隆与序列分析

黄鳝DEAD-box家族PL10基因的克隆与序列分析 Molecular Cloning and Sequence Analysis of DEAD-box Family Gene PL10 from Monopterus albus 彭茂宇 PENG Mao-yu~1 1 2 3 first-author 宋平 SONG Ping~1 1 2 3 桂建芳 GUI Jian-fang~2 1 2 3 武汉大学生命科学学院/发育生物学教育部重点实验室 武汉大学生命科学学院/发育生物学教育部重点实验室 College of Life Sciences,Wuhan University/Key Laboratory of MOE for Developmental Biology College of Life Sciences,Wuhan University/Key Laboratory of MOE for Developmental Biology 中国科学院水生生物研究所,湖北,武汉,430072 中国科学院水生生物研究所,湖北,武汉,430072 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences 淡水生态与生物技术国家重点实验室 淡水生态与生物技术国家重点实验室 通过PCR克隆的方法,从黄鳝（Monopterus albus）中得到两个PL10 基因的cDNA片段Mo PL10A和Mo PL10B,长度均为1.127 kb,推测其编码375个氨基酸的蛋白片段.结合其他PL10类同源物序列,对这两条cDNA进行了分析和初步的功能推测.根据此片段的氨基酸序列构建的系统发育树与形态分类结果一致.在不同组织中的RT PCR结果表明Mo PL10A和Mo PL10B的mRNA在各组织中的分布有差异. Two cDNA fragments identified as Mo PL10A and Mo PL10B were cloned from Monopterus albus by PCR method.They are both 1127bp nucleotides in length and encode two putative proteins of 375 amino acids.The sequences of these two cDNA were compared with the PL10 homology genes in other species.NJ tree based on the amino acid sequence was composed and it was accorded with that of the classical systematics.The RT-PCR essay exhibited an distinction of mRNA levels in the different tissues between Mo PL10A and Mo PL10B. 黄鳝 PL10 DEAD-box RNA解旋酶 基因克隆 序列分析 系统发育树 Monopterus albus PL10 DEAD-box RNA helicase molecular cloning sequence analysis phylogeny tree Q785 国家自然科学基金（30270675）;国家863计划资助项目（2001AA222071） 2005-02-01 2021-04-01 2