水溶性壳聚糖浸种对小麦幼苗抗性相关酶活性的影响

水溶性壳聚糖浸种对小麦幼苗抗性相关酶活性的影响 Effects of Soaking-Seed with Chitosan Aqueous Solution on Activities of Resistance Associated Enzyme from Wheat Seedling 孟祥红 MENG Xiang-hong 1 2 first-author 刘成圣 LIU Cheng-sheng 1 2 于乐军 YU Le-jun 1 2 陈西广 CHENG Xi-guang 1 2 黄全中 HUANG Quan-zhong 1 2 中国海洋大学海洋生命学院 中国海洋大学海洋生命学院 College of Marine Life Sciences, Ocean University of China College of Marine Life Sciences, Ocean University of China 中国海洋大学海洋生命学院,山东,青岛,266003 中国海洋大学海洋生命学院,山东,青岛,266003 利用0.5 g/L的5 种水溶性壳聚糖分别对小麦进行浸种处理,在幼苗发育的5 个时期对叶内苯丙氨酸解氨酶、多酚氧化酶、过氧化物酶和超氧化物歧化酶的活性进行测定.壳聚糖处理降低叶内苯丙氨酸解氨酶活性.多酚氧化酶活性变化不显著,初期酶活性较对照高,随幼苗发育先降后升.壳聚糖浸种处理显著提高叶内过氧化物酶和超氧化物歧化酶活性.5种分子量中以10×103的壳聚糖C浸种处理小麦幼苗叶内抗性相关酶活性强. In this papers, wheat seeds were soaked in chitosan aqueous solution（0.5 g/L） with different molecule, respectively. Activities of PAL, PPO, POD and SOD of wheat seedling leaves from different stages were measured. The results show that PAL activity is slightly lower than control in developing stages, however, PPO activity is individually higher, lower and higher at corresponding preliminary, medium and final developing stage than controls. The results also indicate that POD and SOD activities is obviously higher in 15 d and 20 d than controls. Comparative experiments illuminate the moderate molecule chitosan （10×10 3 ） solution occurs relatively remarkable influence upon the activities of resistance associated enzyme. 小麦 壳聚糖 苯丙氨酸解氨酶 多酚氧化酶 超氧化物岐化酶 过氧化物酶 wheat chitosan phenlanineammonialyase（PAL） polyphenoloxidase（PPO） peroxidase （POD） superoxide dismutase（SOD） S435.12 2005-02-01 2021-04-01 2