单分子层修饰生物芯片基片的电化学研究

单分子层修饰生物芯片基片的电化学研究 Electrochemistry Study on Monolayers Modified Biochips 梁振普 LIANG Zhen-pu~1 1 2 3 4 5 6 7 8 first-author 宋安东 SONG An-dong~1 1 2 3 4 5 6 7 8 Michael Mecklenburg Michael Mecklenburg~2 1 2 3 4 5 6 7 8 Bengt Danielsson Bengt Danielsson~3 1 2 3 4 5 6 7 8 谢斌 XIE Bin~3 1 2 3 4 5 6 7 8 河南农业大学生命科学学院 河南农业大学生命科学学院 College of Life Sciences, Henan Agricultural University College of Life Sciences, Henan Agricultural University BT Biomedical Technology AB BT Biomedical Technology AB BT Biomedical Technology AB,Lund BT Biomedical Technology AB,Lund Department of Pureand Applied Biochemistry Department of Pureand Applied Biochemistry Department of Pure and Applied Biochemistry, Lund University, Lund Department of Pure and Applied Biochemistry, Lund University, Lund Lund University Lund University Lund,22100 Lund,22100 ,Sweden ,Sweden Sweden,河南,郑州,450002 Sweden,河南,郑州,450002 构建了一种亲和素单分子层修饰的电化学分析生物芯片基片,并且以二茂铁和亚铁氰化钾为指示剂对该基片的电化学性能进行了研究.循环伏安实验结果表明, 单分子层的存在增强了二茂铁在基片上的响应电流,二茂铁可以作为该基片的高效电子媒介体,实验结果具有良好的重现性和一致性,基片产生的背景噪声可以忽略不计.文中对这些结果产生的原因进行了讨论. A biotin-avidin modified biochip has been constructed, and its electrochemistry characteristics were studied with K 4 Fe（CN） 6 .3H 2 O and ferrocene as the indicators by cyclic voltammograms. Electrochemical experiments showed that this biochip has high response signal, reproducibility,uniformity and low background signal; the ferrocene can be used as high efficiency electron medium. And these results were discussed in this paper. 生物芯片 单分子层 生物素亲和素系统 电化学 biochips monolayers biotin-avidin system electrochemistry Q819 国家863计划资助项目（2001AA514020 02） 2005-02-01 2021-04-01 2