漆酶催化二氰基氢醌类化合物反应的微量热研究

漆酶催化二氰基氢醌类化合物反应的微量热研究 MICROCALORIMETRIC STUDIES OF LACCASE-CATALYTIC REACTION WITH HYDROQUINONE DERIVATIVES 吴鼎泉 Wu Dingquan 1 2 first-author 王光辉 Wang Guanghu 1 2 张磊 Zhang Lei 1 2 杜予民 Du Yumin 1 2 屈松生 Qu Shongsheng 1 2 武汉大学化学系 武汉大学化学系 Department of Chemistry,Wuhan University Department of Chemistry,Wuhan University 武汉大学化学系,武汉,430072 武汉大学化学系,武汉,430072 用微量热法测量了30℃、pH为4.0时漆酶与底物2,3-二氰基-5,6-二氯-1,4-苯二酚、2,3-二氰基5,6-二溴-1,4-苯二酚、2,3-二氰基-5,6-二碘-1,4-苯二酚催化反应的米氏常数K M ,速率常数k,结合能△G 0 和活化自由能。同时计算了漆酶与2,3-二氰基-5,6-二氯-1,4-苯二酚在30℃、pH=4.0时的反应活性。对实验结果进行了讨论。 The michaelis constants ,the rate constants, Gibbs binding energies and Gibbs activation energies of laccase catalytic reaction with 2,3-dicyan-5,6-dichlorohydroquinone、 2, 3-dicyan-5, 6-dibromohydroquinone and 2, 3-dicyan-5, 6-diiodohydroquinone were deter-mined by LKB-2107 microcalorimeter system at 30℃ and pH = 4. 0. The activity of laccase reaction with 2,3-dicyan-5,6-dichlorohydroquinone was calculated and the experimental re-sult was discussed. 漆酶 微量热法 热动力学 Laccase microcalorimetry thermokinetics O643.3 国家自然科学基金资助的课题 1994-06-01 2021-04-01 6