产碱性蛋白酶地衣芽孢杆菌N₁₆抗噬菌体菌株筛选及噬菌体PG₃特性的研究

产碱性蛋白酶地衣芽孢杆菌N 16 抗噬菌体菌株筛选及噬菌体PG 3 特性的研究 THE SELECTION OF A PHAGE RESISTANT BACILLUS LICHENIFORMIS N 16 PRODUCING ALKALINE PROTEASA AND THE CHARACTERIZATION OF PHAGE PG 3 李广武 1 2 3 4 first-author 杨朝晖 1 2 3 4 岳海峰 1 2 3 4 李雁 1 2 3 4 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 华中师范大学生命科学学院 华中师范大学生命科学学院 河南新乡师范学院生物学系 河南新乡师范学院生物学系 武汉大学生命科学学院华中师范大学生命科学学院河南新乡师范学院生物学系 武汉大学生命科学学院华中师范大学生命科学学院河南新乡师范学院生物学系 报道了噬菌体ＰＧ３对产碱性蛋白酶地衣芽孢杆菌Ｎ１６（Ｂ．ＬｉｃｈｅｎｉｆｏｒｍｉｓＮ１６）反复感染，多次进行自然筛选，获得８株对噬菌体ＰＧ３具有稳定抗性的菌株，其产酶水平有的比出发菌株Ｎ１６高１０％左右．同时对噬菌体ＰＧ３的生物学特性进行了某些研究．实验表明，噬菌体ＰＧ３宿主范围比较广泛，不仅作用于地衣芽孢杆菌的许多株，而且也作用某些其他芽孢杆菌，如巨大芽孢杆菌，苏芸金芽孢杆菌等．此外，在ｐＨ３～１１范围内有较好的稳定性；经６０℃，５ｍｉｎ热处理均失活． Eight strains of Bacillus licheniformis N,, resistant to phage PG3 were obtained through repeated natural selection. The activity of alkaline protease produced by these strains was about 10% higher than the original strain. The characteristics of phage PG3 were also studied. Results indicated that the host range of this phage was relatively wide. Many strains of B. licheniformis and other species of Bacillus such as B. megaterium, B.thuringiensis etc. were also susceptible to phage PG3. The phage was relatively stable in the PH ranging from 3 to 11 and it was killed at 60℃ in 5 min. 地衣芽孢杆菌 噬菌体PG＿3 碱性蛋白酶 Bacillus licheniformis phage PG3 alkaline protease Q939.124.6 国家七·五科技攻关项目 1995-06-01 2021-04-01 6