细胞质雄性不育小麦抽穗期阳离子过氧化物酶同工酶的研究

细胞质雄性不育小麦抽穗期阳离子过氧化物酶同工酶的研究 STUDY ON THE CATION ISOPEROXIDASE OF CYTOPLASMIC MALE STERILE WHEAT IN HEADING STAGE 张花 1 first-author 何之常 1 徐乃瑜 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 选用Ｃ－型、Ｇ－型、Ｍ－型、Ｍｔ２－型４种细胞质类型的７个不育系小麦为材料，以其保持系中国春作对照，用低ｐＨ值（ｐＨ４．６）提取酶液，采用正极和负极向垂直板聚丙烯酸胺凝胶电泳，对抽穗期时、穗和花药的阳离子过氧化物酶同工酶进行了研究．结果表明：花药中不育系和保持系之间无论是正极向还是负极向的过氧化物酶差异均显著．负极向的过氧化物酶不育系比保持系多了两条带且酶带显色更深，反映其酶活性更强；正极向的过氧化物酶中，不育系也比保持系多了两条带，但没有保持系特有的４条酶带（Ａ７～Ａ１０），并且快带也只有一条．花药中阳离子过氧化物酶同工酶谱带数增多，活性增强，可能是小麦雄性不育发生的原因之一． Using seven kinds of four types （C,G,M,Mt2）cytoplasmic male sterile wheat as materials, and their maintainer Chinese Spring as contrast, the canon isoperoxidase of leaves, ears and anthers during heading stage was studied.Enzyme was extracted in the medium of PH4. 6. Electrophoresis was carried towards both cathode and anode. Results showed that there were both obvious difference of isoperoxidase towards cathode and anode between the sterile lines and the maintainer in anthers.lsoperoxidase towards cathode had two more bands in the sterile lines than in the maintainer,and the isoperoxidase bands of sterile lines were thicker,this showed that the activity of peroxidase of the sterile lines was higher than the maintainer. Isoperoxidase towards anode of the sterile lines had two more bands too. The maintainer had four special bands and two fastband of isoperoxidase towards anode,but the sterile lines had not the four special bands and had only one fast-band. More isozyme bands alld higher activity of catlon peroxldase in anthers was probably associated with the cause of male sterility. 小麦 细胞质雄性不育 阳离子过氧化物酶同工酶 wheat cytoplasmic male sterile cation isoperoxidase Q554.6 国家基础性研究攀登计划资助的课题 1995-06-01 2021-04-01 6