极端嗜盐菌冻干保藏及其质检稳定性的研究

极端嗜盐菌冻干保藏及其质检稳定性的研究 STUDIES ON THE PRESERVATION OF FREEZE-DRYING AND THE STABILITY OF PLASMIDS AFTER STORAGE FOR EXTREME HALOPILIC BACTERIA 郑从义 1 2 3 first-author 屈三甫 1 2 3 陶天申 1 2 3 沈萍 1 2 3 武汉大学中国典型培养物保藏中心 武汉大学中国典型培养物保藏中心 China Center for Type Culture Collection, Whhan University,Wuhan 430072,China)Shen ping China Center for Type Culture Collection, Whhan University,Wuhan 430072,China)Shen ping 武汉大学生命科学学院 武汉大学生命科学学院 武汉大学中国典型培养物保藏中心武汉大学生命科学学院 武汉大学中国典型培养物保藏中心武汉大学生命科学学院 采用优化组合保护剂冷冻干燥２０株极端嗜盐菌，于４℃冷库保存２年，经复水测定，全部菌株保持较高存活性．用ＴＥＮＳ法对其中９株菌Ｒ１，ＲＦ１，Ｊ７，Ｓ９，Ｔ４～１，Ｊ６，Ｒ６～１，Ｒ６～７和Ｒ６～５作质粒稳定性检测，含质粒的菌株ＲＦ１，Ｊ７，Ｓ９，Ｔ４～１，Ｒ６～５于４℃保存２年后，质粒稳定存在；而菌株Ｒ１，Ｊ８，Ｒ６～１和Ｒ６～７冻干保存前后均末检测到质粒．结果表明：优化组合保护剂不仅能有效地用于极端嗜盐菌的冷冻干燥保藏，还能保持宿主质粒的稳定性． strains of extreme halophllic bacteria were preserved by freeze-drying in the optimal protectant medium. The viability of these strains and the stability of plasmids for 9 strains of them. H. halobium R1, RF1, J7, S9, R6-1, R6-5, J8, R6-7 and T4-1 were determined after storage at 4℃ for periods up to 2 years. The results showed that all strains tested maintained better viability and plasmids in strains RF1,J7,S9,R6-5and T4-1 still existed stably except strain R1, J8, R6-1and R6-7, in which plasmid was not found originally. These indicated that our optimal protectant medium was an effective protectant of freeze-drying for preserying extreme halophilic bacteria carrying plasmids for a long preiod. 极端嗜盐菌 冷冻干燥 最佳保护剂 质粒稳定性 extreme halophilic bacteria freeze-drying optimal protectant medium plasmid stability Q939.104 1995-06-01 2021-04-01 6