烟草脱外壁花粉的制备

烟草脱外壁花粉的制备 PREPARATION OF EXINE-DETACHED POLLEN IN NICOTIANA TABACUM 夏惠君 1 first-author 周嫦 1 杨弘远 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 烟草脱外壁花粉的制备夏惠君，周嫦，杨弘远（武汉大学生命科学学院，武汉，４３００７２）关键词脱外壁花粉，制备，烟草中图法分类号Ｑ９４４．４２花粉粒由外壁、内壁与原生质体３部分组成．有关花粉生物学的研究已有报道［‘·‘ｊ．近年来，花粉原生质体的研究也取得... A method of preparing exine-detached pollen in Nicotiana tabacum was established.Anthers containing early-middle binucleate pollen were cold-pretreated at 4～6℃ for 7～ 14days, and were suspended in 0. 3 mol· L-1 sucrose solution for 2 days. During this process,the exine of most pollen grains dehisced. Then they were transferred into an enzyme solution containing 1 % cellulase, 1 % pectinase, 0. 1 % pectolyase, 1 mol· L-1 mannitol, 0. 3mol·L-1 sorbitol, 0. 5 % potassium dextran sulphate and K3 medium macro elements. After 15～20 min enzymatic maceration, the exine was detached resulting in the release of exine-detached pollen. Factors affecting preparation of exine-detached pollen were investigated, including cold-pretreatment, osmoticum concentration and enzymes used. The exine-detached pollen as an experimental system may not only be helpful in studying the biological function of exine and intine, but also provide a new receptor system for genetic transformation. 脱外壁花粉 制备 烟草 exine-detached pollen preparation Nicotiana tabacum S572 高等学校博士点专项研究基金，国家自然科学基金 1995-06-01 2021-04-01 6