紫云英根瘤菌5个互补结瘤菌株所分离到的重组质粒外源片段的酶切图谱

紫云英根瘤菌5个互补结瘤菌株所分离到的重组质粒外源片段的酶切图谱 CLEAVING MAPS OF FIVE RECOMBINANT PLASMIDS ISOLATED FROM COMPLEMENTARY NODULATION STRAINS IN RHIZOBIUM HUAKUII 农广 1 2 3 4 first-author 张忠明 1 2 3 4 胡福荣 1 2 3 4 陈华癸 1 2 3 4 李立家 1 2 3 4 华中农业大学生命科学技术学院 华中农业大学生命科学技术学院 Laboratory of Agro-Microbiology, Huazhong Agricultural University Laboratory of Agro-Microbiology, Huazhong Agricultural University 武汉大学生命科学学院 武汉大学生命科学学院 广州中山大学 广州中山大学 华中农业大学生命科学技术学院武汉大学生命科学学院广州中山大学 华中农业大学生命科学技术学院武汉大学生命科学学院广州中山大学 对来源于紫云英根瘤菌７６５３Ｒ总ＤＮＡ基因文库的５个互补结瘤菌株所分离到的重组质粒ｐＮＲ１０２，ｐＮＲ１０３，ｐＮＲ１０８，ｐＮＲ２０３和ｐＮＲ２１３，ＥｃｏＲⅠ酶切表明它们分别携带有一个４．６８，５．０１，５．０４，５．１０或９．３８ｋｂ的外源ＤＮＡ片段．选用１１种内切酶进行单、双酶切分析，建立了重组质粒外源片段的酶切图谱，５个质粒都具有１．７ｋｂ的ＥｃｏＲⅠ－ＢｇｌⅡ片段和１．９ｋｂ的ＥｃｏＲⅠ－ＳａｃⅠ片段． Five recombinant plasmids which came from total DNA genes library of Rhizobium huakuii strain 7653R were isolated from the complementary nodulation strains R102,R103, R108, R203 and R213. They were named as PNR102, PNR103, pNr108, PNR203,PNR213 and contained respectively a 4. 68, 5. 01, 5. 04, 5. 10 or 9. 38 kb foreign fragmentwith EcoR I digestion. Their cleaving maps were made by analysis of single or double restriction endonucleases. The results showed that all recombinant plasmids contained a 1. 7kb EcoR Ⅰ-Bgl Ⅱ fragment and a 1. 9 kb EcoR Ⅰ-Sac Ⅰfragment. 重组质粒 内切酶 酶切图谱 recombinant plasmid restriction endonuclease cleaving map Q939.114 国家“攀登计划”资助 1995-04-01 2021-04-01 4