细胞脂质分析和DNA分子杂交在短状杆菌分类学研究中的作用

细胞脂质分析和DNA分子杂交在短状杆菌分类学研究中的作用 THE ROLE OF ANALYSIS OF CELLULAR LIPIDS AND DNA HYBRIDIZATION IN TAXONOMIC STUDY OF BRACHYBACTERIUM 方呈祥 Fang Chengxiang 1 2 3 first-author 横田明 1 2 3 武内真理子 1 2 3 武汉大学生命科学学院 武汉大学生命科学学院 School of Life Science,Wuhan University,Wuhan, China, 430072)Akira Yokota School of Life Science,Wuhan University,Wuhan, China, 430072)Akira Yokota 日本大阪发酵研究所 日本大阪发酵研究所 Mariko Takeuchi Mariko Takeuchi 武汉大学生命科学学院日本大阪发酵研究所 武汉大学生命科学学院日本大阪发酵研究所 利用薄层层析和气相色谱测定１２株细菌的脂肪酸组份，通过ＵＰＧＭＡ聚类分析绘制其树状图谱；用双相薄层层析获得特征性的极性酯图谱；经光敏生物素非放射性标记ＤＮＡ进行分子杂交测定实验菌株间的ＤＮＡ同源值．一致的结果表明，１２株菌株应分别划归短状杆菌属的３个种，其中有２个被命名为新种，即ＢｒａｃｈｙｂａｃｔｅｒｉｕｍｃｏｎｇｌｏｍｅｒａｔｕｍＩＦＯ１５４７２Ｔ和Ｂ．ｐａｒａｃｏｎｇｌｏｍｅｒａｔｕｍＩＦＯ１５２２４Ｔ．应用上述方法，能有效地对难以从形态特征、生理生化特性确定其分类位置的短状杆菌进一步进行分类 To determine the compositor of lipids of 12 bacteria by the methods of a thinlayer chromatography and of a gas chromatography,euclidian distance of fatty acids was produced by a UPGMA cluster analysis. The diagnostic polar lipid patterns were obtained by two-dimensional thin-layer chromatography. DNA/DNA molecular hybridization was carried out with photobiotinylated DNA for eximation of DNA homologic values. The identical results revealed that 12 strains should be divided into 3 species. Two of them are 2 new species of Brachybacterium conglomeratus IFO 15472 and B. paraconglomeratus IFO 15224 respectively.Depend on the methods,we have classified effectively the bacteria,which it is difficult to determine their taxonomic positions with morphological,physiological and biochemical characterizations. 聚类分析 树状图谱 光敏生物素 短状杆菌属 cluster analysis euclidian distance photobiotin Brachybacterium Q523 日本松前国际友好基金 1995-02-01 2021-04-01 2