玉帘的组织和原生质体培养

玉帘的组织和原生质体培养 TISSUE AND PROTOPLAST CULTURE IN ZEPHYRANTHES CANDIDA HERB 何龙飞 1 first-author 周嫦 1 武汉大学生命科学学院 武汉大学生命科学学院 School of Life Scienc, Wuhan University School of Life Scienc, Wuhan University 玉帘不同外植体培养，诱导出胚性、中间型和非胚性３种类型的愈伤组织，并再生植株．由胚性愈伤组织与叶肉细胞分离出原生质体，进行培养，启动了细胞分裂并形成了多细胞团．同时，还研究了影响玉帘组织培养以及原生质体分离和培养的诸因素． This paper deals with tissue and protoplast culture of Zephyranthes candida Herb. Calli were induced from various explants and plantlets regenerated from calli.The induced calli could be divided into three types:embryogenic, intermediate-type and nonembryogenic callus. The protoplasts derived from embryogenic callus and mesophyll were induced to cell division and cell cluster formation. The factors influencing tissue and protoplast culture were studied as well. 玉帘 组织培养 原生质体培养 愈伤组织 植株再生 Zephyranthes candida tissue culture protoplast culture callus plant regeneration Q949.718.2 国家自然科学基金(61672102) 1995-02-01 2021-04-01 2