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1. 江汉大学化学与环境工程学院
2. 武汉大学化学与分子科学学院
3. 武汉大学化学与分子科学学院,湖北,武汉,430056
4. ,湖北,武汉,430072
纸质出版日期:2006-02-01,
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[1]郑琦,曹启花,蔡汝秀.催化荧光法研究辅酶NADH的抗氧化性能[J].武汉大学学报(理学版),2006(02):133-136.
ZHENG QI~1, CAO QIHUA~2, CAI RUXIU~2. Studies on the Antioxidative Properties of NADH with Spectrofluorimetry. [J]. 2006, (2): 133-136.
[1]郑琦,曹启花,蔡汝秀.催化荧光法研究辅酶NADH的抗氧化性能[J].武汉大学学报(理学版),2006(02):133-136. DOI:
ZHENG QI~1, CAO QIHUA~2, CAI RUXIU~2. Studies on the Antioxidative Properties of NADH with Spectrofluorimetry. [J]. 2006, (2): 133-136. DOI:
采用催化荧光法观察了3种不同类型的活性氧物质(H2O2
.OH
ONOO-)与辅酶NADH的相互作用.研究表明
NADH具有明显的清除活性氧的功能
且对不同类型活性氧的清除作用表现不同:对.OH的清除作用最为迅速
对H2O2
ONOO-的清除作用则较为缓慢.进一步的研究发现
生物体内存在的肌红蛋白因具有过氧化物酶的特性可使清除H2O2的反应大大加速.
The interactions between NADH(nicotinamide adenine dinucleotide) and three kinds of ROS(reactive oxygen species)
i.e.H
2
O
·OH and ONOO
-
were studied.It is found that NADH is capable of scavenging ROS
and the scavenging efficiency is varied toward different objects.Among the three kinds of ROS
OH is eliminated the most rapidly
and H
and ONOO
are eliminated relatively slower.Further studies show that myoglobin
which is an in vivo biomolecule
is able to accelerate the scavenging of H
by NADH notably due to its peroxidatic activity.
催化荧光法NADH抗氧化
spectrofluorimetryNADH(nicotinamide adenine dinucleotide)antioxidation
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