In DOP-PCR experiment

amplification product often generate in negative control.The measures preventing contamination in common and the optimization for reaction’s system and condition can not erase this phenomenon completely.By sublimation、cloning and sequencing for amplification product

it is found that they are generated not because of the contamination but the degeneracy of primer.The degenerate sequences pair each other and generate the primer polymer.The concentration of primer is very important to the amplification product’s yield.So the optimizing study on primer’s concentration of reaction condition is carried on in this experiment as well.And the result shows that 2.2 μmol/L is better concentration to reduce the yield of amplification product.