Emodin liposome was prepared by the reverse evaporating method.The entrapment efficiency and particle size of the emodin liposome were determined.Through the examination of dose-and time-effect by trypan blue method

the toxicity of emdoin liposome in Hep-2 cells was studied

and growth curve of liposome-treated Hep-2 cells was drawn.The results show that the emodin liposome prepared by the reverse evaporating method has a high entrapment efficiency of 90.6%

and its particle size is 0.02-0.1 μm.The results of cytotoxicity study indicate that the liposome exhibits evident toxicity in Hep-2 cells and has dose-and time-effect

as the concentration of emodin liposome raised from 0.48 mg·L

-1

to 14.48 mg·L

-1

the ratio of dead cells increased from 23.15% to 94.5%

and 48 h IC

50

of the liposome is 4.43 mg·L

-1

after 12 h liposome with a concentration of 7.24 mg·L

-1

was acted on Hep-2 cells

the ratio of dead cells increased to over 95%.Comparing the growth curve of liposome-treated cells with normal Hep-2 cells

the growth curve of liposome-treated cells declined evidently and this result indicates that emodin liposome exhibits control effect on Hep-2 cells growth.