漆酶的活性测定及催化单取代Fc—R氧化反应

漆酶的活性测定及催化单取代Fc—R氧化反应 THE DETERMINATION OF LACCASE ACTIVITY AND ITS CATALYTIC OXIDATION TO MONOSUBSTITUTE FERROCENE DERIVATIVES 季立才 Ji Licai 1 2 3 first-author 胡培植 Hu Peizhi 1 2 3 汤贵生 Tang Guisheng 1 2 3 刘惠英 Liu Hueying 1 2 3 武汉大学生命科学学院　化学系 武汉大学生命科学学院　化学系 School of Life Sciences,Wuhan University School of Life Sciences,Wuhan University 江西南昌化工原料厂 江西南昌化工原料厂 武汉大学生命科学学院　化学系江西南昌化工原料厂 武汉大学生命科学学院　化学系江西南昌化工原料厂 在二乙二醇单丁醚（ＤＧＢＥ）／磷酸盐缓冲液体系中，以二茂铁（Ｆｃ—Ｈ）为底物，用分光光度法测定湖北毛坝漆树漆酶的活性无副产物干扰．该方法简便、准确、易行．考察了漆树漆酶对２９个单取代二茂铁衍生物（Ｆｃ—Ｒ）和左旋多巴的催化氧化反应，发现了漆酶１４个新的底物，并讨论了它们的构效关系 The determination of activity of Rhus vernicifera laccase from Hubei Maoba have not interference of by product using ferrocene as substrate in the system of diethylene glycol monobutyl ether （DGBE）/phosphate butter by spectrophotometric method.The method is a simple,convenient,accurate and easy.It was investigated that Rhus vernicifera laccase catalyzed oxidation of 29 monosubstitute ferrocene derivatives and left dopa.New 14 substrates of laccase were found,and the relationship of theirs structure and function is discussed. 酶活性 漆树漆酶 分光光度法 二茂铁 底物 enzyme activity rhus vernicifera laccase spectrophotometric method ferrocene substrate O643.3 1996-04-01 2021-04-01 4