玉米（Zea　mays　L．）cdc2基因的染色体原位杂交物理定位

玉米（Zea　mays　L．）cdc2基因的染色体原位杂交物理定位 THE PHYSICAL LOCATION OF THE MAIZE CDC2 GENE BY IN SITU HYBRIDIZATION 任南 Ren Nan 1 first-author 宋运淳 Song Yuchun 1 毕学知 Bi Xuerzhi 1 刘立华 Liu Lihua 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 首次报道了玉米低拷贝ｃｄｃ２基因非放射性标记的探针染色体原位杂交的物理定位．供试探针为玉米，ｐ３４ｃｄｃ２的ｃＤＮＡ克隆ｃｄｃ２ＺｍＡ，片段长度仅为１．３ｋｂ．原位杂交检测结果表明，该基因位置在第４染色体及第９染色体长臂，同时在第８染色体长臂亦有杂交信号检出．３处信号检出位置距着丝粒距离和长臂长度百分比分别为５４．７９±２．５１６，８７．９４±１．５３７和２５．８６±０．８５６在第４、９和８染色体上信号检出率分别为２４．６％，１２．３％和６．６％．本文还就非放射原位杂交技术以及ｃｄｃ２基因在玉米基因组中的物理位置与其功能间的关系作了探讨 A digoxigeninlabeled in situ hybridization technique was first used to physically map a low copy gene cdc2 in maize. The cDNA probe, cdc2 ZmA, was hybridized onto the long arm of the fourth and the ninth chromosomes. The percent distance from centromere to detection site were respetively 54.79±2.516 and 87.94±1.537.This fulllength cDNA clone was only 1.3kb . The average detection rate of in situ hybridization was 32.3% , which was higher than that observed with biotin labeling technique in other studies. The results demonstrated that gene cdc2 had multiple sites in maize. The techniques of chromosome preparation and mapping low or single copy genes with ISH in plants have been discussed. 玉米 cdc2基因 染色体原位杂交 地高辛标记 maize Gene cdc2 Chromosome in situ hybridization Digoxigenin labeling Q7 国家自然科学基金,国家教委博士点基金 1996-06-01 2021-04-01 6