产碱性聚半乳糖醛酸酶嗜碱细菌抗利福平高产菌株的选育

产碱性聚半乳糖醛酸酶嗜碱细菌抗利福平高产菌株的选育 HIGH PRODUCERS OF POLYGALACTURNASE SELECTED FROM MUTANTS RESISTANT TO RIFAMPIN IN ALKALOPHILIC BACLLUS SP. NTT33 曹军卫 Cao Junwei 1 first-author 孙卫华 Sun Weihua 1 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 分离得到产碱性果胶酶嗜碱芽孢杆菌ＮＴＴ３３，其聚半乳糖醛酸酶的产生强烈地受葡萄糖的代谢阻遏，对利福平敏感．通过抗利福平自然突变法，从出发菌株ＮＴＴ３３中筛选抗利福平突变株．采用透明圈法初步筛选出在含葡萄糖和不含葡萄糖果胶培养基上同时产生透明圈的菌株，进一步测定其产酶进程曲线，最后筛选出两株（ＮＴＴ３３－ｃｓ５２，ＮＴＴ３３－ｃｓ３０１）产聚半乳糖醛酸酶活力高的菌株，其酶活力比出发菌株提高５０％～２８０％，并且在葡萄糖存在时，酶活力更高．可以认为这两株菌消除了葡萄糖的代谢阻遏作用 Synthesis of Polygalacturonase in alkalophilic Bacillus sp. strain NTT33 is repressed strongly by glucose and is sensitive to rifampin. Several mutants resistant to rifampin were screened. Clearance zones produced by the mutants were contrasted in pectin medium and pectin added glucose medium. The strains with similar P/C value in both PM and GPM were selected. Time courses of enzyme activity of the strains were determined in liquid media OM and GOM. Last, the two strains （NTT33 cs52 and NTT33 cs301）that produced the high activity of polygalacturonase were screened. Their activities of polygalacturonase are more higher than that of the wild type strain NTT33. The highest enzyme activity has increased by 81.8%. It was shown that their glucose catabolic repressions were eliminated. 嗜碱细菌 聚半乳糖醛酸酶 利福平 抗葡萄糖代谢阻遏 alkalophilic bacteria polygalacturonase rifampin resistant to glucose catabolic repression Q556.2 1996-06-01 2021-04-01 6