ELISA法以单一单克隆抗－HBe测定e系统的研究

ELISA法以单一单克隆抗－HBe测定e系统的研究 AN ELISA FOR MEASUREMENT OF HBeAg AND HBeAb 刘永平 1 first-author 刘子夜 1 赵伟光 1 岳莉莉 1 黄永秀 1 齐义鹏 1 武汉大学病毒研究所 武汉大学病毒研究所 Institute of Virology, Wuhan University Institute of Virology, Wuhan University 制备了一株ＨＢｅ单抗细胞株，同时用于包被和酶标，以ＥＬＩＳＡ法测定ｅ抗原及病人血清中抗－ＨＢｅ获得成功． In recent products, sandwich-ELISA and neutralization-ELISA have beenestablished separately for the determination of HBeAg and HBeAb employing two anti-HBemonoclonal antibodies or one monoclonal antibody and one polyclonal antiserum. Monoclonalantibody from one cell clone was purified and used forcoating and peroxidase-conjugating applied in the ELISA Kit for HBeAg and HBeAb. That one monoclonal antibody was availablefor antigenbinding was demonstrated by measurement of HBeAg and human serum of clinicpatients. HBeAg 单克隆抗体 ELISA HBeAg monoclonal antibody ELISA R373.21 1996-02-01 2021-04-01 2